Dimorphic Mechanism on cAMP Mediated Signal Pathway in Mucor circinelloides

Abstract

Mucor circinelloides is a dimorphic fungus that is a non-pathogen strain belonging to zygomycetes. In this research, a part of hypothetical mechanism on yeast-like cell induction of M. circinelloides in CO2 atmosphere was reported from the viewpoint of gene expression. To explain the relation between the change and the expressions of some genes involved in morphological changes of the strain, these were analyzed on the filamentous and yeast cell by real-time qPCR. The compared genes were Nce103, Ras3, Cyr1, Pde, and Efg1 encoding carbonic anhydrase, GTPase, adenylate cyclase, phosphodiesterase, and elongation factor G1, respectively. In anaerobic grown yeast cell with 70%N2 + 30%CO2, the Nce103 and Ras3 gene expressions decreased to 24 h whereas that of the filamentous cell increased. However, a downstream gene of Cyr1 expression level in the yeast cell was higher than that of filamentous cell. A lower level of Pde in the yeast cell than that of the filamentous cell indicated intracellular cAMP accumulation. The actual cAMP in the yeast cell remained whereas that of the filamentous cell decreased with cultivation. The Efg1 expression level controlling hyphal elongation was suppressed in the yeast cell. The intracellular cAMP accumulation and Efg1 expression regulate hyphal elongation or yeast forming.

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Data Availability

The datasets during and/or analyzed during the current study are available from the corresponding author on a reasonable request.

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Correspondence to Kazuhiro Hoshino.

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The presenting author of this manuscript in ACB2019 is Maki Moriwaki-Takano.

The title of the presentation is “Elucidation of dimorphic mechanism on cAMP mediated signal pathway in Mucor circinelloides” as P1-036.

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Moriwaki-Takano, M., Iwakura, R. & Hoshino, K. Dimorphic Mechanism on cAMP Mediated Signal Pathway in Mucor circinelloides. Appl Biochem Biotechnol 193, 1252–1265 (2021). https://doi.org/10.1007/s12010-020-03342-6

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Keywords

  • Fungal dimorphism
  • Morphology
  • CO2
  • cAMP
  • Elongation factor G1
  • Mucor circinelloides