The most preferred decellularization technique in creating bioscaffolds for complex organs such as kidneys is through detergent perfusion. Detergents such as sodium dodecyl sulfate (SDS) flow to the kidneys to remove cells but using this technique alone requires long treatment times. Coupling this technique with sonication treatment decreases decellularization time but may cause damages in the microarchitecture of the kidney. This study evaluated the effects of decellularization parameters specifically SDS concentration (0.25%, 0.625%, and 1.0%wt/vol), flowrate (15, 30, and 45 mL/min), and sonicator power (0, 60, and 120 W) on the length of time needed to produce acellular and intact bioscaffolds. Decellularization was carried out by perfusing SDS to the renal artery of the cadaveric porcine kidney while exposed to sonication treatment. Results showed that a significant decrease in decellularization time was observed in producing acellular scaffold when perfusion decellularization was coupled with sonication. In addition, SDS concentration, SDS flowrate, and sonicator power had significant effects on the decellularization time while only sonicator power had a significant effect on the microarchitecture integrity of the scaffold. Lastly, H&E results showed that the produced bioscaffold showed complete cell removal with only minimal to moderate disruptions on the microarchitecture of the kidney.
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This work was funded by the Commission on Higher Education (CHED) Discovery-Applied Research and Extension for Trans/Inter-disciplinary Opportunities (DARETO) Project Research Grant, the Department of Science and Technology Engineering Research and Development for Technology (DOST-ERDT), and the De La Salle University Research and Coordination Office (URCO).
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The presenting author of this manuscript in ACB2019 is Tosha Mae Manalastas.
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Manalastas, T.M., Dugos, N., Ramos, G. et al. Effect of Decellularization Parameters on the Efficient Production of Kidney Bioscaffolds. Appl Biochem Biotechnol 193, 1239–1251 (2021). https://doi.org/10.1007/s12010-020-03338-2
- Detergent perfusion
- Extracellular matrix