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Genome-Scale Characterization of Fungal Phytases and a Comparative Study Between Beta-Propeller Phytases and Histidine Acid Phosphatases

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Abstract

This work intended to prospect new phytase-producing organisms. In silico genomic analyses allowed the selection of twelve potential phytase-producing fungi. Based on gene sequence, it was possible to identify four well-defined groups of phytate-degrading enzymes: esterase-like, β-propeller phytases (βPP), phosphoglycerate mutase-like, and phytases of the histidine acid phosphatases (HAP) family. Analysis of the predicted genes encoding phytases belonging to the HAP family and βPP phytases and in silico characterization of these enzymes indicated divergence among the catalytic activities. Predicted fungal βPP phytases exhibited higher molecular mass (around 77 kDa) probably due to the epidermal growth factor-like domain. Twelve sequences of phytases contained signal peptides, of which seven were classified as HAP and five as βPP phytases, while ten sequences were predicted as phytases secreted by non-classical pathways. These fungi were grown in liquid or semi-solid medium, and the fungal enzymatic extracts were evaluated for their ability to hydrolyze sodium phytate at 50 °C and pH ranging from 2.0 to 9.0. Seven fungi were identified as phytase producers based on phosphate release under enzyme assay conditions. Results obtained from in silico analyses combining experimental enzymatic activities suggest that some selected fungi could secrete βPP phytases and HAP phytases.

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Acknowledgments

We acknowledge the Brazilian institutions CAPES for the scholarship granted to the first author, FAPEMIG, and CNPq for the resources provided to complete this study.

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Authors and Affiliations

  1. Department of Biochemistry and Molecular Biology, Federal University of Viçosa, Av. PH Rolfs, s/n, Viçosa, MG, 36570-900, Brazil

    Roberta Corsino Ferreira, Murillo Peterlini Tavares, Túlio Morgan, Yan da Silva Clevelares, Marina Quádrio Raposo Branco Rodrigues, Tiago Antônio de Oliveira Mendes & Valéria Monteze Guimarães

  2. Department of Microbiology, Federal University of Viçosa, Viçosa, Brazil

    Maria Catarina Megumi Kasuya

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  1. Roberta Corsino Ferreira
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  2. Murillo Peterlini Tavares
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  3. Túlio Morgan
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  4. Yan da Silva Clevelares
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Correspondence to Valéria Monteze Guimarães.

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Ferreira, R.C., Tavares, M.P., Morgan, T. et al. Genome-Scale Characterization of Fungal Phytases and a Comparative Study Between Beta-Propeller Phytases and Histidine Acid Phosphatases. Appl Biochem Biotechnol 192, 296–312 (2020). https://doi.org/10.1007/s12010-020-03309-7

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