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Biodegradation of Reactive Orange 16 Dye in Microbial Fuel Cell: An Innovative Way to Minimize Waste Along with Electricity Production

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Abstract

Microbial fuel cell (MFC) is an attractive green technology which harnesses the power of microorganism for the production of electricity along with bioremediation of waste. However, the bioremediation of the high concentration of dye wastewater in MFC remains unclear. In present study, double-chambered MFC inoculated with mixed bacterial consortium was used for bioremediation of reactive orange 16 (RO-16) dyes at a very high and variable concentration range of 100 to 1000 ppm. Maximum voltage was obtained for 100 ppm of dye and was found to be 0.5791 V along with a power density of 0.0851 W/m3. Till 500 ppm concentration of dye COD removal efficiency remains in range of 40 to 100% thereafter it decreases. The maximum concentration of CO2 was found to be 2% at 1000 ppm which confirms the biodegradation phenomena in MFC. Kinetics of biodegradation of reactive orange 16 were studied using Haldane inhibitory kinetic model and kinetic constants μmax, Ks, and Ki were calculated and found to be 0.417 day−1, 206.2 ppm, and 447.12 ppm respectively. The experimental results showed inhibitory condition in the MFC after 500 ppm and it was supported by the value of inhibitory kinetic constant Ki = 447.12 ppm. This study opened the possibility of bioremediation of dyes at high concentrations in MFCs.

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Funding

This work was supported by the Department of Chemical Engineering and Technology, Centre of Advanced Study, Indian Institute of Technology, BHU, and Center for Energy and Resource Development (CERD), IIT, BHU, Varanasi 221005, India.

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  1. Department of Chemical Engineering & Technology, Centre of Advanced Study, Indian Institute of Technology, BHU, Varanasi, 221005, India

    Amrita Shahi, Birendra Nath Rai & Ram Sharan Singh

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  1. Amrita Shahi
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  3. Ram Sharan Singh
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Correspondence to Ram Sharan Singh.

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Since this article does not contain any human study, so there is no need of taking consent to anyone. This work is totally based on lab experiments performed on bioreactor made in the lab by the researchers.

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Highlights

• First-time reactive orange 16 dye was taken as a substrate for the microbial fuel cells.

• Almost 100% color removal was observed at 100 ppm of dye within 24 h.

• The high concentration of dye (500 ppm) is successfully removed in MFC in 24 h.

• If the residence time of dye is increased from 24 to 72 h then 600 to 800 ppm of dye can also be removed.

• The inhibition study was done with the Haldane model and kinetic constants were calculated and supported the experimental observations.

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Shahi, A., Rai, B.N. & Singh, R.S. Biodegradation of Reactive Orange 16 Dye in Microbial Fuel Cell: An Innovative Way to Minimize Waste Along with Electricity Production. Appl Biochem Biotechnol 192, 196–210 (2020). https://doi.org/10.1007/s12010-020-03306-w

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