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Preparation of Biocompatible Liquid Marbles Stabilized by Food-Grade Stearate Microparticle for Aerobic Bacteria Cultivation

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Abstract

Liquid marble (LM), a non-stick drop coated with micro- or nano-scale particles, has great potential in a wide range of applications. LMs have an advantageous feature in which gas or vapor can freely transport through their particle shell; therefore, it makes them an ideal candidate to be utilized as microbioreactor containing aerobic microorganisms. In this study, safer and more biocompatible LMs were successfully prepared using a food-grade calcium stearate microparticle as a stabilizer. As the volume of core liquid increased, the height of LM increased and reached a constant value, as a similar trend has been reported in conventional LMs. The drying rate curve of the LMs confirmed that the LMs have a similar pattern with the drying of typical wet powders. The drying rate depended on the salt species in the core solution and the environmental humidity. For instance, in the case of MgCl2, by changing humidity from 40 to 80% RH, the lifetime of LMs (time in which the LM dried completely) was increased to about 900 min. This is nearly three times longer than those have no salt and at 40% RH. Model aerobic bacteria Bacillus subtilis has actively proliferated inside the LM during 24-h incubation. Comparing with the test tube cultivations under O2-rich stationary or O2 rich–shaken conditions, the cultivation in the LM system showed a higher proliferation than the test tube systems. As a conclusion, we demonstrated that the calcium stearate LM system would be an ideal candidate for safer and easily available microbioreactor containing aerobic bacteria.

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Funding

This research was supported by the JSPS KAKENHI Grant Number JP18K04830.

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Authors and Affiliations

  1. Department of Materials Science & Chemical Engineering, Faculty of Advanced Engineering, National Institute of Technology, Nara College, Yamato-Koriyama, Nara, 639-1080, Japan

    Shoma Tanaka & Kazumitsu Naoe

  2. R&D Division, Taihei Chemical Industrial Co. Ltd., Ikaruga, Nara, 636-0104, Japan

    Hiroaki Okano & Nobuyuki Matsuda

  3. Department of Nutrition & Life Science, Faculty of Applied Bioscience, Kanagawa Institute of Technology, Atsugi, Kanagawa, 243-0292, Japan

    Jun Sawai

  4. Department of Food Science & Technology, College of Bioresource Science, Nihon University, Fujisawa, Kanagawa, 252-8510, Japan

    Masanao Imai

Authors
  1. Shoma Tanaka
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  2. Hiroaki Okano
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  3. Nobuyuki Matsuda
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  4. Jun Sawai
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  5. Kazumitsu Naoe
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Correspondence to Kazumitsu Naoe.

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Tanaka, S., Okano, H., Matsuda, N. et al. Preparation of Biocompatible Liquid Marbles Stabilized by Food-Grade Stearate Microparticle for Aerobic Bacteria Cultivation. Appl Biochem Biotechnol 191, 1684–1694 (2020). https://doi.org/10.1007/s12010-020-03299-6

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  • DOI: https://doi.org/10.1007/s12010-020-03299-6

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