Abstract
Garcinia mangostana pericarp is a good source of natural antioxidants with numerous functional properties. The conventional approaches for the recovery of antioxidants from Garcinia mangostana pericarp require long processing time and high temperature, which may cause degradation or loss of bioactivity of antioxidants, and often result in low recovery efficiency. In this study, the extraction of antioxidants from Garcinia mangostana pericarp was investigated using a polyethylene glycol (PEG)/citrate aqueous biphasic system (ABS) with the addition of surfactants. The optimum condition for the recovery of antioxidants was achieved in PEG 1000/citrate ABS of pH 8 with tie-line length (TLL) of 48.3% (w/w), volume ratio (VR) of 1.6, 0.2% (w/w) sample loading and addition of 1.0% (w/w) Tween 85. The antioxidants were recovered in the PEG-rich top phase with a high K value of 18.23 ± 0.33 and a recovery yield of 92.01% ± 0.09. The findings suggested that the addition of surfactants to polymer/salt ABS can enhance the recovery of antioxidants from Garcinia mangostana pericarps by conserving the antioxidative properties.
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This work was financially supported by the UCSI University Pioneer Scientist Incentive Funds under grant number [PROJ-2019-In-FAS-064] and Industry Grant (Proj-In-ALPHA-003) from Alpha Active Industries Sdn Bhd, Selangor, Malaysia; with laboratory support from Faculty of Applied Sciences, UCSI University, Malaysia.
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Ng, HS., Kee, P.E., Tan, G.YT. et al. Surfactant as an Additive for the Recovery of Potent Antioxidants from Garcinia mangostana Pericarps Using a Polymer/Salt Aqueous Biphasic System. Appl Biochem Biotechnol 191, 273–283 (2020). https://doi.org/10.1007/s12010-020-03284-z
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DOI: https://doi.org/10.1007/s12010-020-03284-z