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A Sensitive Electrochemical Ascorbic Acid Sensor Using Glassy Carbon Electrode Modified by Molybdenite with Electrodeposited Methylene Blue

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Abstract

A non-enzymatic amperometric sensor using natural molybdenite (MLN) electrodeposited with methylene blue (MB) has been fabricated and characterized and its analytical performances were investigated for the determination of ascorbic acid (AA). The surface morphology of the electrode modified by electrodeposited MB was studied by use of the Advanced Mineral Identification and Characterization System (AMICS) and laser confocal high-temperature scanning microscope (LCSM). The poly(MB) and MLN immobilized sensor showed good stability, reproducibility, sensitivity, and selectivity. It exhibited a linear performance range from 3 to 1000 μM, with a lower detection limit of 0.083 μM (signal/noise = 3) and short response time (< 5 s). No obvious decrease in the current was observed after 20 days storage. The methodology reproducibility of this sensor was 2.6%. It showed good anti-interference ability for the potential interfering compounds. The poly(MB) film not only can enhance the electron-transfer rate but also increase the lifetime of the sensor. This study demonstrated the applicability of natural molybdenite for the fabrication of non-enzymatic electrochemical AA sensor.

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Acknowledgments

The authors gratefully thank to the financial support from University of Science and Technology Liaoning (No. 2019TD01) which made this work possible.

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Authors and Affiliations

  1. School of Chemical Engineering, University of Science and Technology Liaoning, 185 Qianshan Middle Road, High-tech zone, Anshan, 114051, Liaoning, China

    Yan Zhang, Yue Wang & Zhiqiang Zhang

  2. Department of Life Science and Green Chemistry, Faculty of Engineering, Saitama Institute of Technology, 1690 Fusaiji, Fukaya, Saitama, 369-0293, Japan

    Yasushi Hasebe

  3. School of Mineral Engineering, University of Science and Technology Liaoning, 185 Qianshan Middle Road, High-tech zone, Anshan, 114051, Liaoning, China

    Dongping Tao

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  1. Yan Zhang
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Correspondence to Yue Wang or Dongping Tao.

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Zhang, Y., Wang, Y., Hasebe, Y. et al. A Sensitive Electrochemical Ascorbic Acid Sensor Using Glassy Carbon Electrode Modified by Molybdenite with Electrodeposited Methylene Blue. Appl Biochem Biotechnol 191, 1533–1544 (2020). https://doi.org/10.1007/s12010-020-03255-4

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