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Novel Copper Bearing Schiff Bases with Photodynamic Anti-Inflammatory and Anti-Microbial Activities

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Abstract

Schiff bases and their copper complexes have been previously studied for their anti-inflammatory, anti-tumor as well as anti-microbial activities. Schiff bases can be derivatized to gain photoluminiscence capacity. This property of the schiff bases enables the transfer of the electrons upon absorption of the light at a specific wavelength. In this study, we exploited this attribute of novel copper bearing schiff bases and tested their photodynamic biological activities. These compounds exerted photodynamic anti-inflammatory activities on the in vitro activated mammalian macrophages. Compared with salicylic acid control groups, these novel schiff bases had stronger activity which became more prominent with photo-induction. Moreover, they also had anti-microbial activity on gram negative bacteria E.coli and gram positive bacteria S.aureus.This anti-microbial activity was stronger than that of Neomycin on both bacterial strains. Our results suggest their potential use as anti-inflammatory and anti-microbial agents both in the dark as well as after photo-induction.

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Abbreviations

TNFα:

tumor necrosis factor α

LPS:

lipopolysaccharide

ELISA:

enzyme linked immunosorbent assay

PDT:

photodynamic therapy

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Acknowledgments

We greatly appreciate the guidance and support of Dr. Juan Anguita from CIC Biogune, Spain.

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Authors and Affiliations

  1. Department of Biotechnology, Faculty of Arts and Science, Mersin University, 33110, Mersin, Turkey

    Furkan Ayaz & Burcu Demirbag

  2. Department of Chemistry, Faculty of Arts and Science, Cukurova University, 01330, Adana, Turkey

    Ilyas Gonul

  3. Department of Energy Systems Engineering, Faculty of Technology, Tarsus University, 33400, Tarsus, Turkey

    Kasim Ocakoglu

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  1. Furkan Ayaz
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  2. Ilyas Gonul
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  4. Kasim Ocakoglu
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All authors contributed to the manuscript writing process equally and approved the final version of the manuscript.

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Correspondence to Furkan Ayaz or Ilyas Gonul.

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Highlights

• Schiff base derivatives had anti-inflammatory activity.

• They decreased pro-inflammatory cytokine secretion by macrophages.

• They lacked cytotoxicity or immunostimulant activity.

• Addition of copper to the structure created a photo responsive anti-inflammatory agent

• These compounds also had strong dark anti-microbial activities.

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Ayaz, F., Gonul, I., Demirbag, B. et al. Novel Copper Bearing Schiff Bases with Photodynamic Anti-Inflammatory and Anti-Microbial Activities. Appl Biochem Biotechnol 191, 716–727 (2020). https://doi.org/10.1007/s12010-019-03223-7

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