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Magnetic Cross-Linked Enzyme Aggregates of Aspergillus oryzae ST11 Lipase Using Polyacrylonitrile Coated Magnetic Nanoparticles for Biodiesel Production

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Abstract

The biodiesel production by enzymatic catalysis is the environmentally friendly production process. In this work, the polyacrylonitrile coated magnetic nanoparticles were prepared and used as a core supporter for producing magnetic cross-linked enzyme aggregates (mCLEAs) from Aspergillus oryzae ST11 lipase with the co-feeding of bovine serum albumin (BSA). The highest immobilized lipase activity was 0.09 U/mg-support or 81.7% of recovered activity. The resulting mCLEAs exhibited the desired characteristics; it had improved the stabilities for pH, temperature, as well as storage comparing to free enzyme. Moreover, when this biocatalyst was used for biodiesel production at the optimal conditions (30% w/w of mCLEAs, 30% w/w of water content and stepwise addition of methanol), the biodiesel conversion was 95% within 24 h of reaction, while one-step addition of methanol produced 81% conversion. The mCLEAs could be reused for 5 cycles and retained the biodiesel conversion higher than 60%.

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Acknowledgments

This research was supported by the joint research fund between Prince of Songkla University and the Thailand Research Fund through the Royal Golden Jubilee Scholarship Ph.D. Program (Grant No. PHD/0321/2552).

Funding

This study was funded by the joint research fund between Prince of Songkla University and the Thailand Research Fund through the Royal Golden Jubilee Scholarship Ph.D. Program (Grant No. PHD/0321/2552).

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  1. Department of Industrial Biotechnology, Faculty of Agro-Industry, Prince of Songkla University, Hat Yai, 90110, Thailand

    Pattarapon Paitaid & Aran H-Kittikun

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  1. Pattarapon Paitaid
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  2. Aran H-Kittikun
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Correspondence to Pattarapon Paitaid.

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Paitaid, P., H-Kittikun, A. Magnetic Cross-Linked Enzyme Aggregates of Aspergillus oryzae ST11 Lipase Using Polyacrylonitrile Coated Magnetic Nanoparticles for Biodiesel Production. Appl Biochem Biotechnol 190, 1319–1332 (2020). https://doi.org/10.1007/s12010-019-03196-7

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