Correction to: Phytase Immobilization on Hydroxyapatite Nanoparticles Improves Its Properties for Use in Animal Feed

Correction to: Applied Biochemistry and Biotechnology

https://doi.org/10.1007/s12010-019-03116-9

In the original version of this article, under Calculation of Immobilization Parameters heading, the presentation of the equations are incorrect. The correct presentation of the equations are given below:

Calculation of immobilization parameters

The percentage immobilization yield (IY) was calculated using the equation:

$$ IY\left(\%\right)=1-\frac{\left[{P}_{supernatant1}\right]+\left[{P}_{supernatant2}\right]\ }{\left[{P}_{control}\right]}\times 100, $$

where P_supernatant1 and P_supernatant2 (mg mL^-1) are the protein concentrations for supernatant 1 (obtained after the first wash) and supernatant 2 (obtained after the second wash), respectively, and P_control (mg mL^-1) is the protein concentration for the control (soluble enzyme). The enzymatic activity that was offered to the support (A_Of) was calculated using the equation:

$$ {A}_{of}\left(\frac{IU}{g\ support}\right)=\frac{A_{solubleenzyme}\times volume\ of\ enzyme\ offered\ \left( in\ mL\right)}{mass\ of\ support\ \left( in\ g\right)}, $$

where A_{soluble enzyme} (IU mL^-1) is the activity of the free enzyme. The theoretically immobilized activity (A_TI, in IU g^-1 support) was obtained as the product of the activity offered to the support (A_Of) and IY × 100^-1. The recovered activity (RA) of the immobilized enzyme was calculated as follows:

$$ RA\left(\%\right)=\raisebox{1ex}{${A}_{DE}$}\!\left/ \!\raisebox{-1ex}{${A}_{of}$}\right.\times 100, $$

where A_DE is the activity of the derivative (IU g^-1 support).