Abstract
This approach was constructed to appraise the therapeutic effectiveness of a single i.v. dose of osteoblasts generated from co-culturing BM-MSCs with nano-HA, Pt-NPs, or Pt-HA-nanocomposite in osteoporotic rats. MSCs were grown, propagated in culture, and characterized. The effect of the suggested nanoplatforms on the survival, osteogenic differentiation, and mineralization of BM-MSCs was assessed by MTT assay, real-time PCR analysis, and Alizarin red S staining, respectively. Thereafter, the generated osteoblasts were employed for the treatment of ovariectomized rats. Our results revealed that the selected nanoplatforms upregulate the expression of osteogenic differentiation related genes (Runx-2 and BMP-2) significantly and enhance calcium deposition in BM-MSCs after 7 and 21 days, respectively, whereas the in vivo study validated that the infusion of the generated osteoblasts considerably downturn serum BALP, BSP, and SOST levels; upswing OSX level; and regain femur bone mineralization and histoarchitecture. Conclusively, the outcomes of this work provide scientific evidence that transplanting osteoblasts derived from differentiation of BM-MSCs in the presence of nanoplatforms in ovariectomized rats restores bone remodeling balance which constitutes a new hope for the treatment of osteoporosis.
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Abbreviations
- BALP:
-
Bone alkaline phosphatase
- BM-MSCs:
-
Bone marrow derived mesenchymal stem cells
- BMP-2:
-
Bone morphogenetic protein 2
- BSP:
-
Bone sialoprotein
- Nano-HA:
-
Nanohydroxyapatite
- OSX:
-
Osterix
- Pt-NPs:
-
Platinum nanoparticles
- Pt-HA-nanocomposite:
-
Platinum-hydroxyapatite-nanocomposite
- Runx-2:
-
Runt-related transcription factor 2
- SOST:
-
Sclerostin
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The authors gratefully acknowledge the financial support of the National Research Centre, Egypt (Grant no. P100528) and the technical support of the Science and Technology Development Fund (STDF), Egypt, through Capacity Building program (Grant no. 4880). Also, the authors express sincere appreciation to Prof. Adel Bakeer kholoussy, Professor of Pathology, Faculty of Veterinary Medicine, Cairo University for his kind cooperation in conducting histological examination in this study.
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This work was financially supported by the National Research Centre, Egypt (grant no. P100528).
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Aglan, H.A., Ahmed, H.H., Mahmoud, N.S. et al. Nanotechnological Applications Hold a Pivotal Position in Boosting Stem Cells Osteogenic Activity: In Vitro and In Vivo Studies. Appl Biochem Biotechnol 190, 551–573 (2020). https://doi.org/10.1007/s12010-019-03105-y
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DOI: https://doi.org/10.1007/s12010-019-03105-y