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Surface-Displayed Thermostable Candida rugosa Lipase 1 for Docosahexaenoic Acid Enrichment

Applied Biochemistry and Biotechnology volume 190, pages 218–231 (2020)Cite this article

Abstract

Yeast surface display has emerged as a viable approach for self-immobilization enzyme as whole-cell catalysts. Herein, we displayed Candida rugosa lipase 1 (CRL LIP1) on the cell wall of Pichia pastoris for docosahexaenoic acid (DHA) enrichment in algae oil. After a 96-h culture, the displayed CRL LIP1 achieved the highest activity (380 ± 2.8 U/g) for hydrolyzing olive oil under optimal pH (7.5) and temperature (45 °C) conditions. Additionally, we improved the thermal stability of displayed LIP1, enabling retention of 50% of its initial bioactivity following 6 h of incubation at 45 °C. Furthermore, the content of DHA enhanced from 40.61% in original algae oil to 50.44% in glyceride, resulting in a 1.24-fold increase in yield. The displayed CRL LIP1 exhibited an improved thermal stability and a high degree of bioactivity toward its native macromolecule substrates algae oil and olive oil, thereby expanding its potential for industrial applications in fields of food and pharmaceutical. These results suggested that surface display provides an effective strategy for simultaneous convenient expression and target protein immobilization.

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Acknowledgments

All authors would like to thank the Analytical and Testing Center of Huazhong University of Science and Technology for their valuable assistances in the gas chromatography–mass spectrometry measurements.

Funding

This research was funded by the National Natural Science Foundation of China (grant number: NSFC31170078), the National High Technology Research and Development Program of China (grant numbers: 2013AA065805 and 2014AA093510), the National Natural Science Foundation of Hubei Province (grant number: 2015CFA085), and the Fundamental Research Funds for HUST (grant numbers: 2014QN119 and 2014NY007).

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Author notes

  1. Li Xu and Xiao Xiao contributed equally to this work.

Affiliations

  1. Key Laboratory of Molecular Biophysics of the Ministry of Education, College of Life Science and Technology, Huazhong University of Science and Technology, Luo-Yu Road #1037, Wuhan, 430074, China

    Li Xu, Xiao Xiao, Fei Wang, Yaojia He, Xiaoxu Yang, Jinrui Hu, Zhe Feng & Yunjun Yan

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  1. Li Xu
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Correspondence to Li Xu.

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Xu, L., Xiao, X., Wang, F. et al. Surface-Displayed Thermostable Candida rugosa Lipase 1 for Docosahexaenoic Acid Enrichment. Appl Biochem Biotechnol 190, 218–231 (2020). https://doi.org/10.1007/s12010-019-03077-z

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Keywords

  • Candida rugosa lipase 1
  • Pichia pastoris
  • Cell surface displaying
  • Whole-cell catalyst
  • Docosahexaenoic acid