Definition of Liquid and Powder Cellulase Formulations Using Domestic Wastewater in Bubble Column Reactor

  • Nelson Libardi
  • Carlos Ricardo Soccol
  • Valcineide O. A. Tanobe
  • Luciana Porto de Souza VandenbergheEmail author


Raw domestic wastewater was used as a culture medium for cellulase production in a bubble column reactor (6.2 UFP/mL, 64.6 U/L h) using the strain Trichoderma harzianum TRIC03-LPBII. Cellulases presented optimum pH and temperature between 4 and 5 and 50 and 70 °C, respectively. Enzymatic extract was concentrated through ultrafiltration and then a cellulolytic formulation was prepared with the addition of sorbitol (50% w/v) and benzoic acid (0.05% w/v). High cellulase stability of around 100% was reached after 30 days at 4 °C. The concentrated extract was also dried in a spray-dryer with the addition of maltodextrin at 20% (w/v), resulting in powder enzymatic formulation with 85% stability after 60 days. With these characteristics, the liquid and powder cellulase products have potential to be used in different industrial applications.


Trichoderma harzianum FPase β-Glucosidases Bubble column bioreactor Domestic wastewater Enzyme formulation 



This study was financed in part by Coordenação de Aperfeiçoamento de Pessoal de Nível Superior-Brasil (CAPES) (Finance code 001), Conselho Nacional de Desenvolvimento Científico e Tecnológico-Brasil (CNPq), and internal funds of Federal University of Paraná.

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interest.


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© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Departamento de Engenharia de Bioprocessos e BiotecnologiaUniversidade Federal do Paraná—UFPRCuritibaBrazil

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