With growing concern about the possible risks and side effects of antibiotic drugs, more and more natural products with antibacterial activity are studied as the substitutes. In this paper, the antibacterial activity of hydroquinone and arbutin in Ainsliaea bonatii was investigated, which both displayed relatively strong antibacterial activity against Staphylococcus aureus (SA), methicillin-resistant S. aureus (MRSA), and extended spectrum β-lactamase S. aureus (ESBL-SA). The antibacterial mechanism of hydroquinone had been explored by scanning electron microscopy (SEM), alkaline phosphatase (AKP), and bacterial extracellular protein leakage. Results showed that hydroquinone could destroy the bacterial cell wall and membrane, increase permeability, lead leakage of intracellular substance affect synthesis of protein, and influence expression of genes.
This is a preview of subscription content, access via your institution.
Buy single article
Instant access to the full article PDF.
Tax calculation will be finalised during checkout.
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
Tax calculation will be finalised during checkout.
Olson, M. E., King, J. M., Yahr, T. L., & Horswill, A. R. (2013). Sialic Acid Catabolism in Staphylococcus aureus. Journal of Bacteriology, 195(8), 1779–1788.
Editorial Board of Chinese Academy of Sciences Flora. (1996). Flora of China. Beijing: Science Press.
Wang, W., & Kang, W. Y. (2013).Chemical constituents from Ainsliaea bonatii, Chinese Pharmaceutical Journal, 48(3), 174–176.
Zhao, M. Q., Shen, H. Y., Pan, W., Wang, J. Y., Li, Y. G., & Chang, Y. (2011). Chin Anim Husb Vet Med, 38(5), 177–181.
Jiang, X. F., Hong, X. H., Sun, J. Y., Chen, Y. L., & Ni, Y. X. (2002).Analysis of beta-lactamase in multi-resistant Pseudomonas aeruginosa, Chinese Journal of Microbiology and Immunology, 22(4), 443–446.
Wang, M. G. (2006). Research about the drug resistance and plasmid-mediated resistance mechanism of quinolones, National Medical Journal of China, 86(9), 645–647.
McKeegan, K. S., Borges-Walmsley, M. I., & Walmsley, A. R. (2003). The structure and function of drug pumps: an update. Trends in Microbiology, 11(1), 21–29.
Keith, P. (2001). Outer membranes and efflux: the path to multidrug resistance in Gram-negative bacteria, Current Opinion in Microbiology, 4(5), 500–508.
Kalkhambkar, R. G., Waters, S. N., & Laali, K. K. (2011). Highly efficient synthesis of amides via Ritter chemistry with ionic liquids. Tetrahedron Letters, 52(8), 867–871.
Ali, N. A. A., Jülich, W. D., Kusnick, C., & Lindequist, U. (2001). Screening of Yemeni medicinal plants for antibacterial and cytotoxic activities. Journal of Ethnopharmacology, 74(2), 173–179.
Liu, G., Ma, Y. M., & Zhang, H. C. (2013).Isolation, identification and antimicrobial activities of endophytic fungi from Cephalotaxus fortunei , Chinese Pharmaceutical Journal, 48(3), 165–170.
Li, C. Q., Zhao, L., Yang, Y. T., & Kang, W. Y. (2011). Antimicrobial activity of Salvia miltiorrhiza and different processed products, Chinese Traditional Patent Medicine., 33(11), 1948–1951.
Liu, H. Y., Zhao, D., Chang, J., Yan, L., Zhao, F. J., Wu, Y. C., Xu, T., Gong, T., Chen, L., He, N. N., Wu, Y., Han, S. Q., & Qu, D. (2014). Efficacy of novel antibacterial compounds targeting histidine kinase YycG protein. Applied Microbiology and Biotechnology, 98(13), 6003–6013.
He, N., Zhou, J., Hu, M. Y., Ma, C. Y., & Kang, W. Y. (2018). The mechanism of antibacterial activity of corylifolinin against three clinical bacteria from Psoralen corylifolia L. Open Chemistry, 16(1), 882–889.
Gu, Y. P., Liu, H. Z., & Luo, P. (2014).Antimicrobial effects of chitosan on the main pathogens from urinary tract infection in vitro, Applied Chemical Industry., 43(7), 1184–1188.
Xu, Y. Y., Cai, S. S., & Yu, J. (2014). Scavenging ability for nitrite and antibacterial mechanism of phytosterol from Cortex mori, Modern Food Science and Technology., 30(2), 53–57.
Lan, W. Q., Xie, J., Hou, W. F., & Li, D. W. (2012). Antimicrobial activity and mechanism of complex biological fresh-keeping agents against Staphylococcus sciuri, Natural Product Research and Development, 24(6), 741–746 753.
Lee, H. J., Choi, G. J., & Cho, K. Y. (1998). Correlation of Lipid Peroxidation inBotrytiscinereaCaused by Dicarboximide Fungicides with Their Fungicidal Activity. Journal of Agricultural and Food Chemistry, 46(2), 737–740.
He, N., Wang, P. Q., Wang, P. Y., Ma, C. Y., & Kang, W. Y. (2018). Antibacterial mechanism of chelerythrine isolated from root of Toddalia asiatica (Linn) Lam. BMC Complementary and Alternative Medicine, 18(1), 261–269.
Hara, S., & Yamakawa, M. (1995). Bombyx mori. Journal of Biochemistry, 270(50), 29923–229927.
Jing, Y. J., Hao, Y. J., Qu, H., Shan, Y., Li, D. S., & Du, R. Q. (2006). Preliminary studies on antibacterial mechanism and analysis of antibacterial activity of chitosans, Chinese Journal of Antibiotics, 31(6), 361–365.
Wang Q. (2011). Master thesis, Liaoning Normal University, Henan, China.
Liu, S. X., Wei, H. P., Cheng, J., & Yang, J. Q. (2012).Studies on antibacterial mechanism of the volatile oils from Eupatorium adenophorum Spreng on Staphylococcus aureus, Chin J Hosp Pharm, 32(21), 1742–1745.
Qiang, W., Wang, H. L., Zhou, C. F., & Suo, Y. R. (2011).Determination of protein contents from Caragana korshinskii Kom. seeds using coomassie brilliant blue g-250 dyeing, Amino Acids and Biotic Resources., 33(3), 74–76.
Yuan, J. L. (2015). Microbiology (9th ed.). Beijing: China Traditional Medicine Press.
We gratefully acknowledge the valuable cooperation of the members of the National Center for Research and Development of Edible Fungus Processing Technology, Henan University, in the experiments.
This work was supported by grant (2017YFC1601400) from the Ministry of Science and Technology of the People’s Republic of China and grant (182102110332) from the Scientific and Technology Department of Henan Province.
Statement of Ethics
We declare that the ethical background of this study was approved by the National Ethical Committee.
Conflict of Interest
The authors declare that they have no conflicts of interest.
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
About this article
Cite this article
Ma, C., He, N., Zhao, Y. et al. Antimicrobial Mechanism of Hydroquinone. Appl Biochem Biotechnol 189, 1291–1303 (2019). https://doi.org/10.1007/s12010-019-03067-1
- Ainsliaea bonatii
- Antibacterial mechanism
- Staphylococcus aureus