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Augmenting Fremyella diplosiphon Cellular Lipid Content and Unsaturated Fatty Acid Methyl Esters Via Sterol Desaturase Gene Overexpression

  • Somayeh Gharaie Fathabad
  • AnithaChristy S. Arumanayagam
  • Behnam Tabatabai
  • Huan Chen
  • Jie Lu
  • Viji SittherEmail author
Article
  • 69 Downloads

Abstract

Cyanobacteria have immense prospective as a platform for renewable energy; however, a major barrier in achieving optimal productivity is the low lipid yield. Fremyella diplosiphon, a model cyanobacterium, is an ideal biofuel agent due to its desirable fatty acid methyl esters (FAMEs). To enhance lipid content, we overexpressed the sterol desaturase (SD) gene in F. diplosiphon B481 wild type by genetic transformation. This effort resulted in a transformant (B481-SD) with a 64-fold increase in the SD gene at the mRNA transcript level, with no loss in growth and pigmentation. The transformant was persistently grown for over 32 generations indicating long-term stability and vitality. We observed 27.3% and 23% increases in total lipid content and unsaturated FAMEs respectively in B481-SD transesterified lipids with methyl octadecadienoate as the most abundant unsaturated component. In addition, we detected an 81% increase in FAME composition in the transformant compared with the wild type. Theoretical physical and chemical properties confirmed a FAME profile with very high cetane number (65.972–67.494) and oxidative stability (50.493–18.66 h) in the engineered strain. Results of the study offer a promising approach to augment F. diplosiphon total lipid content and unsaturated FAMEs, thus paving the way to enhance biofuel capacity of the organism.

Keywords

Alkane Biofuel Cyanobacteria Sterol desaturase Transcript abundance 

Notes

Funding Information

The work was partially supported by the National Institutes of Health (UL1GM118973) awarded to Morgan State University and National Science Foundation (DMR 11-57490 and DMR-16-44779) awarded to the National High Magnetic Field Laboratory and the State of Florida.

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interest.

Supplementary material

12010_2019_3055_MOESM1_ESM.docx (96 kb)
ESM 1 (DOCX 92 kb)

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2019

Authors and Affiliations

  1. 1.Department of BiologyMorgan State UniversityBaltimoreUSA
  2. 2.Department of PathologyMethodist Hospital Research InstituteHoustonUSA
  3. 3.National High Magnetic Field LaboratoryFlorida State UniversityTallahasseeUSA
  4. 4.Future Fuels InstituteFlorida State UniversityTallahasseeUSA

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