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Using Natural Waste Material as a Matrix for the Immobilization of Enzymes: Chicken Eggshell Membrane Powder for β-Galactosidase Immobilization

Applied Biochemistry and Biotechnology volume 187, pages 101–115 (2019)Cite this article

Abstract

Avian eggshell membranes are good candidates as a matrix for immobilization procedures. Chicken eggshell, a waste material available from the poultry industry as a byproduct, is a very safe and cheap raw material. While pieces of eggshell membrane, or even particles from whole eggshell, have been previously used for these purposes, we report here the use of eggshell membrane powder for E. coli β-galactosidase immobilization with glutaraldehyde as cross-linker. A kinetic characterization is provided for eggshell membrane powder-bound enzyme compared to free enzyme. Results show a remarkable similarity between bound and free enzyme and also that the immobilized enzyme is stable and can be reused several times. Moreover, bound enzyme is able to produce glucose from skim milk serum.

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Acknowledgements

This work was supported by FONDECYT 1150681 granted by the Chilean Council for Science and Technology (CONICYT). Special thanks are given to Victor Castro-Fernández (Facultad de Ciencias, Universidad de Chile) for his invaluable help in obtaining and managing structural data and to David Carrino for critical reading of the manuscript.

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  1. Departamento de Ciencias Biológicas Animales, Facultad de Ciencias Veterinarias y Pecuarias, Universidad de Chile, Santa Rosa 11735, La Pintana, Santiago, Chile

    E. Kessi & J. L. Arias

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Correspondence to E. Kessi.

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Kessi, E., Arias, J.L. Using Natural Waste Material as a Matrix for the Immobilization of Enzymes: Chicken Eggshell Membrane Powder for β-Galactosidase Immobilization. Appl Biochem Biotechnol 187, 101–115 (2019). https://doi.org/10.1007/s12010-018-2805-4

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Keywords

  • Eggshell membrane powder
  • Enzyme immobilization
  • β-Galactosidase