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Penicillium purpurogenum Produces a Set of Endoxylanases: Identification, Heterologous Expression, and Characterization of a Fourth Xylanase, XynD, a Novel Enzyme Belonging to Glycoside Hydrolase Family 10

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Abstract

The fungus Penicillium purpurogenum grows on a variety of natural carbon sources and secretes a large number of enzymes which degrade the polysaccharides present in lignocellulose. In this work, the gene coding for a novel endoxylanase has been identified in the genome of the fungus. This gene (xynd) possesses four introns. The cDNA has been expressed in Pichia pastoris and characterized. The enzyme, XynD, belongs to family 10 of the glycoside hydrolases. Mature XynD has a calculated molecular weight of 40,997. It consists of 387 amino acid residues with an N-terminal catalytic module, a linker rich in ser and thr residues, and a C-terminal family 1 carbohydrate-binding module. XynD shows the highest identity (97%) to a putative endoxylanase from Penicillium subrubescens but its highest identity to a biochemically characterized xylanase (XYND from Penicillium funiculosum) is only 68%. The enzyme has a temperature optimum of 60 °C, and it is highly stable in its pH optimum range of 6.5–8.5. XynD is the fourth biochemically characterized endoxylanase from P. purpurogenum, confirming the rich potential of this fungus for lignocellulose biodegradation. XynD, due to its wide pH optimum and stability, may be a useful enzyme in biotechnological procedures related to this biodegradation process.

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Funding

This work has been supported by grants from FONDECYT (1130180) and Universidad Andrés Bello (DI-478-14/R and DI-31-12/R).

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  1. Departamento de Ciencias Biológicas, Universidad Andrés Bello, República 217, Santiago, Chile

    Valentina Echeverría & Jaime Eyzaguirre

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  1. Valentina Echeverría
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  2. Jaime Eyzaguirre
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Correspondence to Jaime Eyzaguirre.

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Echeverría, V., Eyzaguirre, J. Penicillium purpurogenum Produces a Set of Endoxylanases: Identification, Heterologous Expression, and Characterization of a Fourth Xylanase, XynD, a Novel Enzyme Belonging to Glycoside Hydrolase Family 10. Appl Biochem Biotechnol 187, 298–309 (2019). https://doi.org/10.1007/s12010-018-2782-7

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  • DOI: https://doi.org/10.1007/s12010-018-2782-7

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