Applied Biochemistry and Biotechnology

, Volume 186, Issue 2, pp 400–413 | Cite as

Valorization of the Crude Glycerol for Propionic Acid Production Using an Anaerobic Fluidized Bed Reactor with Grounded Tires as Support Material

  • Talita Corrêa Nazareth
  • Aline Gomes de Oliveira Paranhos
  • Lucas Rodrigues Ramos
  • Edson Luiz Silva


This study evaluated the propionic acid (HPr) production from crude glycerol (CG) (5000 mg L−1) in an anaerobic fluidized bed reactor (AFBR). Grounded tire particles (2.8–3.35 mm) were used as support material for microbial adhesion. The reactor was operated with hydraulic retention times (HRT) varying from 8 to 0.5 h under mesophilic (30 °C) conditions. The HPr was the main metabolite produced, increasing in composition from 66.5 to 99.6% by decreasing the HRT from 8 to 0.5 h. Other metabolic products were 1,3-propanediol, with a maximum of 29.4% with an HRT of 6 h, ethanol, acetic, and butyric acids. The decrease in HRT from 8 to 0.5 h decreased the HPr yield, with a maximum of 0.48 ± 0.06 g HPr g COD−1 and an HRT of 6 h, and favored HPr productivity, with a maximum of 4.09 ± 1.24 g L−1 h−1 and HRT of 0.5 h. In the biogas, the H2 content increased from 12.5 to 81.2% by decreasing the HRT from 8 to 0.5 h. These results indicate the potential application of the AFBR for HPr production using an immobilized mixed culture.


Fermentation Hydraulic retention time Organic acids Propionic acid productivity Propionic acid yield 


Funding Information

The authors gratefully acknowledge the financial support of FAPEAM (Amazonas Research Foundation), CAPES (Coordination for the Improvement of Higher Education Personnel), CNPq (National Council for Scientific and Technological Development), and FAPESP (São Paulo Research Foundation).


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Talita Corrêa Nazareth
    • 1
  • Aline Gomes de Oliveira Paranhos
    • 1
  • Lucas Rodrigues Ramos
    • 1
  • Edson Luiz Silva
    • 1
  1. 1.Department of Chemical EngineeringFederal University of São CarlosSão CarlosBrazil

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