Valorization of the Crude Glycerol for Propionic Acid Production Using an Anaerobic Fluidized Bed Reactor with Grounded Tires as Support Material
- 100 Downloads
This study evaluated the propionic acid (HPr) production from crude glycerol (CG) (5000 mg L−1) in an anaerobic fluidized bed reactor (AFBR). Grounded tire particles (2.8–3.35 mm) were used as support material for microbial adhesion. The reactor was operated with hydraulic retention times (HRT) varying from 8 to 0.5 h under mesophilic (30 °C) conditions. The HPr was the main metabolite produced, increasing in composition from 66.5 to 99.6% by decreasing the HRT from 8 to 0.5 h. Other metabolic products were 1,3-propanediol, with a maximum of 29.4% with an HRT of 6 h, ethanol, acetic, and butyric acids. The decrease in HRT from 8 to 0.5 h decreased the HPr yield, with a maximum of 0.48 ± 0.06 g HPr g COD−1 and an HRT of 6 h, and favored HPr productivity, with a maximum of 4.09 ± 1.24 g L−1 h−1 and HRT of 0.5 h. In the biogas, the H2 content increased from 12.5 to 81.2% by decreasing the HRT from 8 to 0.5 h. These results indicate the potential application of the AFBR for HPr production using an immobilized mixed culture.
KeywordsFermentation Hydraulic retention time Organic acids Propionic acid productivity Propionic acid yield
The authors gratefully acknowledge the financial support of FAPEAM (Amazonas Research Foundation), CAPES (Coordination for the Improvement of Higher Education Personnel), CNPq (National Council for Scientific and Technological Development), and FAPESP (São Paulo Research Foundation).
- 2.ANP-National Agency of Oil, Natural Gas and Biofuels (Brazil). (2016) Brazilian Statistical Yearbook of Oil, Natural Gas and Biofuels, 1st ed National Agency of Oil, Natural gas and biofuels, Rio de Janeiro, Brazil (in Portuguese).Google Scholar
- 14.Barros, A. R., Adorno, M. A. T., Sakamoto, I. K., Maintinguer, S. I., Varesche, M. B. A., & Silva, E. L. (2011). Performance evaluation and phylogenetic characterization of anaerobic fluidized bed reactors using ground tire and pet as support materials for biohydrogen production. Bioresource Technology, 102(4), 3840–3847.CrossRefGoogle Scholar
- 16.Walker, M., Zhang, Y., Heaven, S., & Banks, C. (2009). Potential errors in the quantitative evaluation of biogas production in anaerobic digestion process. Bioresource Technology, 100, 116–123.Google Scholar
- 19.American Public Health Association. (2012). Standard methods for the examination for water and wastewater (22nd ed.). Washington: American Water Works Association, Water Environmental Federation.Google Scholar
- 20.van Haandel, A. and van der Lubbe, J. (2011) Handbook biological wastewater treatment: design and optimization of activated sludge systems. 1. ed. Leidschendam: Quist, Netherlands.Google Scholar
- 26.Playne, M. J. (1985). Propionic and butyric acids. In M. Moon-Young (Ed.), Comprehensive biotechnology (pp. 731–759). New York: Pergamon Press.Google Scholar
- 27.Coral, J., Karp, S. G., Vandenberghe, L. P. S., Parada, J. L., Pandey, A., & Soccol, C. R. (2008). Batch fermentation model of propionic acid production by Propionibacterium acidipropionici in different carbon sources. Applied Microbiology and Biotechnology, 151, 333–341.Google Scholar
- 33.Jeris, J. S., & Mccarty, P. L. (1965). The biochemistry of methane fermentation using C14 tracers. Journal of the Water Pollution Control Federation, 37, 178–192.Google Scholar
- 34.Novak, J. T., & Carlson, D. A. (1970). The kinetics of anaerobic long-chain fatty acids degradation. Journal of the Water Pollution Control Federation, 42, 1932–1943.Google Scholar
- 36.Kósmider, A., Drozdzynska, A., Blaszka, K., Leja, K., & Czaczyk, K. (2010). Propionic acid production by Propionibacterium freudenreichii ssp Shermanii using industrial wastes: crude glycerol and whey lactose. Polish Journal of Environmental Studies, 19, 1249–1253.Google Scholar
- 38.Zhang, A., & Yang, S. T. (2009). Propionic acid production from glycerol by metabolically engineered Propionibacterium acidipropionici. Process Biochemistry, 44, 239–259.Google Scholar