Enhanced Bioethanol Production from Potato Peel Waste Via Consolidated Bioprocessing with Statistically Optimized Medium
In this study, an extensive screening was undertaken to isolate some amylolytic microorganisms capable of producing bioethanol from starchy biomass through Consolidated Bioprocessing (CBP). A total of 28 amylolytic microorganisms were isolated, from which 5 isolates were selected based on high α-amylase and glucoamylase activities and identified as Candida wangnamkhiaoensis, Hyphopichia pseudoburtonii (2 isolates), Wickerhamia sp., and Streptomyces drozdowiczii based on 26S rDNA and 16S rDNA sequencing. Wickerhamia sp. showed the highest ethanol production (30.4 g/L) with fermentation yield of 0.3 g ethanol/g starch. Then, a low cost starchy waste, potato peel waste (PPW) was used as a carbon source to produce ethanol by Wickerhamia sp. Finally, in order to obtain maximum ethanol production from PPW, a fermentation medium was statistically designed. The effect of various medium ingredients was evaluated initially by Plackett-Burman design (PBD), where malt extracts, tryptone, and KH2PO4 showed significantly positive effect (p value < 0.05). Using Response Surface Modeling (RSM), 40 g/L (dry basis) PPW and 25 g/L malt extract were found optimum and yielded 21.7 g/L ethanol. This study strongly suggests Wickerhamia sp. as a promising candidate for bioethanol production from starchy biomass, in particular, PPW through CBP.
KeywordsBioethanol Consolidated bioprocessing Wickerhamia sp. Plackett-Burman design Response surface methodology
Response surface modeling
Analysis of variance
Potato peel waste
We thank Md. Kamruzzaman Pramanik and Tabassum Mumtaz from the Microbiology and Industrial Irradiation Division, Institute of Food and Radiation Biology, Atomic Energy Research Establishment, Ganakbari Savar Dhaka, Bangladesh, for providing suggestion for the operation of the bioreactor.
Abdullah-Al- Mahin designed the whole study; Tahmina Hossain and Abdul Bathen Miah performed the experiments; and Tahmina Hossain, Siraje Arif Mahmud, and Abdullah-Al- Mahin drafted the manuscript and analyzed the results.
This work was not supported by any funding.
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Conflict of Interests
The authors declare that they have no conflict of interest.
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