Prominent Study on Surface Properties and Diffusion Coefficient of Urease-Conjugated Magnetite Nanoparticles
- 69 Downloads
Herein, the magnetite nanoparticles (MNs) were prepared by facile solvothermal method and its porous nature was modified using 3-(2-aminoethyl)-3-aminopropyl trimethoxysilane (AEAPS). Magnetite formation, successful amino tagging, and urease conjugation on the surface were confirmed from the presence of certain functional groups in Fourier transform infrared (FT-IR) spectra. Also, nanosize (13.2 nm) and spherical morphology of MNs were evaluated from diffraction patterns and electron micrographs respectively. Lower retentivity and coercivities in magnetization curve revealed the superparamagnetic behavior, and nitrogen adsorption/desorption curves exhibited decrease in its surface porosity. Conductivity measurements showed lower diffusion coefficient (De = 1.9 × 10−17 cm2/min) and higher diffusion with limited hydrolytic reaction in native urease and improved activity of conjugated urease with higher De (12.62 × 10−16 cm2/min). Hence, this study revealed that the surface porous nature of MNs can be altered effectively by amino tagging in order to overcome diffusional limitations thereby enhancing enzyme activity.
KeywordsMagnetite Urease Activity Porosity Diffusion coefficient Diffusional limitations
Compliance with Ethical Standards
Conflict of Interest
The authors declare that they have no conflict of interest.
- 3.Gabrielczyk, J., Duensing, T., Buchholz, S., Schwinges, A., Jordening, H.J. (2018). A comparative study on immobilization of fructosyl transferase in biodegradable polymers by electrospinning. Applied Biochemistry and Biotechnology, 1–16. https://doi.org/10.1007/s12010-018-2694-6
- 4.Zou, B., Chu, Y., Xia, J., Chen, X., Huo, S. (2017). Immobilization of lipase by ionic liquid-modified mesoporous SiO2 adsorption and calcium alginate-embedding method. Applied Biochemistry and Biotechnology, 1–13. https://doi.org/10.1007/s12010-017-2676-0
- 12.Caldas, E. M., Novatzky, D., Deon, M., de Menezes, E. W., Hertz, P. F., Costa, T. M. H., Arenas, L. T., & Benvenutti, E. V. (2017). Pore size effect in the amount of immobilized enzyme for manufacturing carbon ceramic biosensor. Microporous and Mesoporous Materials, 247, 95–102.CrossRefGoogle Scholar
- 21.Seenuvasan, M., Vinodhini, G., Malar, C. G., Balaji, N., & Kumar, K. S. (2017). Magnetic nanoparticles: a versatile carrier for enzymes in bio-processing sectors. IET Nanobiotechnology. https://doi.org/10.1049/iet-nbt.2017.0041.
- 25.Wang, T., Zhang, L. Y., Wang, H. Y., Yang, W. C., Fu, Y. C., Zhou, W. L., Yu, W. T., Xiang, K. S., Sun, Z., Dai, S., & Chai, L. Y. (2013). Controllable synthesis of hierarchical porous Fe3O4 particles mediated by poly(diallyldimethylammonium chloride) and their application in arsenic removal. ACS Applied Materials & Interfaces, 5(23), 12449–12459.CrossRefGoogle Scholar
- 27.Seenuvasan, M., Malar, C. G., Preethi, S., Balaji, N., Iyyappan, J., Kumar, M. A., & Kumar, K. S. (2013). Immobilization of pectinase on co-precipitated magnetic nanoparticles for enhanced stability and activity. Research Journal of Biotechnology, 8, 24–30.Google Scholar
- 28.Seenuvasan, M., Malar, C. G., Preethi, S., Balaji, N., Iyyappan, J., Kumar, M. A., & Kumar, K. S. (2013). Fabrication, characterization and application of pectin degrading Fe3O4-SiO2 nanobiocatalyst. Materials Science & Engineering. C, Materials for Biological Applications, 33(4), 2273–2279.CrossRefGoogle Scholar
- 34.Karimzadeh, I., Dizaji, H. R., & Aghazadeh, M. (2016). Development of a facile and effective electrochemical strategy for preparation of iron oxides (Fe3O4 and γ-Fe2O3) nanoparticles from aqueous and ethanol mediums and in situ PVC coating of Fe3O4 superparamagnetic nanoparticles for biomedical applications. Journal of Magnetism and Magnetic Materials, 416, 81–88.CrossRefGoogle Scholar
- 36.Karimzadeh, I., Aghazadeh, M., Doroudi, T., Ganjali, M. R., & Kolivand, P. H. (2017). Superparamagnetic iron oxide (Fe3O4) nanoparticles coated with PEG/PEI for biomedical applications: a facile and scalable preparation route based on the cathodic electrochemical deposition method. Advances in Physical Chemistry, 2017, 1–7. https://doi.org/10.1155/2017/9437487.CrossRefGoogle Scholar
- 37.Asgari, S., Fakhari, Z., & Berijani, S. (2014). Synthesis and characterization of Fe3O4 magnetic nanoparticles coated with carboxymethyl chitosan grafted sodium methacrylate. Journal Nanostructured, 4, 55–63.Google Scholar
- 38.Akbari, B., Tavadashti, M. P., & Zandrahimi, M. (2011). Particle size characterization of nanoparticles—a practical approach. Iranian Journal of Materials Science and Engineering, 8, 48–56.Google Scholar
- 40.Tanaka, H., Matsumura, M., & Veliky, I.A. (1984). Diffusion characteristics of substrates in Ca-alginate gel beads, Biotechnology and Bioengineering 26, 053–058, 1, 53.Google Scholar