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Prominent Study on Surface Properties and Diffusion Coefficient of Urease-Conjugated Magnetite Nanoparticles

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Abstract

Herein, the magnetite nanoparticles (MNs) were prepared by facile solvothermal method and its porous nature was modified using 3-(2-aminoethyl)-3-aminopropyl trimethoxysilane (AEAPS). Magnetite formation, successful amino tagging, and urease conjugation on the surface were confirmed from the presence of certain functional groups in Fourier transform infrared (FT-IR) spectra. Also, nanosize (13.2 nm) and spherical morphology of MNs were evaluated from diffraction patterns and electron micrographs respectively. Lower retentivity and coercivities in magnetization curve revealed the superparamagnetic behavior, and nitrogen adsorption/desorption curves exhibited decrease in its surface porosity. Conductivity measurements showed lower diffusion coefficient (De = 1.9 × 10−17 cm2/min) and higher diffusion with limited hydrolytic reaction in native urease and improved activity of conjugated urease with higher De (12.62 × 10−16 cm2/min). Hence, this study revealed that the surface porous nature of MNs can be altered effectively by amino tagging in order to overcome diffusional limitations thereby enhancing enzyme activity.

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Correspondence to Muthulingam Seenuvasan or Kannaiyan Sathish Kumar.

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Research Highlights

• One-step facile solvothermal method was used for the magnetite nanoparticle (MNs) preparation.

• The surface porosity of MNs was concealed by successful amino tagging (aMNs).

• Different analytical and instrumental techniques were performed to reveal various physicochemical and surface properties.

• Urease conjugation (U-aMNs) was achieved by glutaraldehyde cross-linkage.

• Conductivity measurements were done to evaluate the diffusion coefficient and co-relate the relation between porosity and diffusional limitations.

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Malar, C.G., Seenuvasan, M. & Kumar, K.S. Prominent Study on Surface Properties and Diffusion Coefficient of Urease-Conjugated Magnetite Nanoparticles. Appl Biochem Biotechnol 186, 174–185 (2018). https://doi.org/10.1007/s12010-018-2719-1

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  • DOI: https://doi.org/10.1007/s12010-018-2719-1

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