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Direct Utilization of Non-pretreated Hydrolytic Liquid of Dried Distiller’s Grains with Solubles for Bio-Ethanol by Rhizopus arrhizus RH 7-13-9#

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Abstract

Bio-ethanol, as an environment friendly and renewable fuel, has gained increasing worldwide attention and can be produced through the fermentation of the carbohydrates or sugar(s) fraction of biomass materials. Here, dried distiller’s grains with solubles (DDGS), a waste in production of bio-ethanol, were applied to prepare acid-hydrolytic liquid and it was directly used as seed culture and fermentation medium without pretreatment. Rhizopus arrhizus RH 7-13-9# cultured in non-pretreated acid-hydrolytic liquid with pH 4.7 for 30 h could utilize the concentrated hydrolytic liquid as well as the hydrolytic liquid mixed with glucose. A high yield of bio-ethanol was obtained. It was proven that common used medium could be replaced by non-pretreated acid-hydrolytic liquid to some extent. The pretreated process of acid-hydrolytic liquid was avoided that decreased feed stock cost and was significant for the utilization of acid-hydrolytic liquid from lignocellulose materials.

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References

  1. Baeyens, J., Kang, Q., Appels, L., Dewil, R., Lv, Y., & Tan, T. (2015). Challenges and opportunities in improving the production of bio-ethanol. Progress in Energy and Combustion Science, 47, 60–88.

    Article  Google Scholar 

  2. Balat, M. (2011). Production of bioethanol from lignocellulosic materials via the biochemical pathway: a review. Energy Conversion and Management, 52(2), 858–875.

    Article  CAS  Google Scholar 

  3. Kang, Q., Appels, L., Baeyens, J., Dewil, R., & Tan, T. (2014). Energy-efficient production of cassava-based bio-ethanol. Advances in Bioscience and Biotechnology, 05(12), 925–939.

    Article  Google Scholar 

  4. Zhang, H. L., Baeyens, J., Degrève, J., & Cacères, G. (2013). Concentrated solar power plants: review and design methodology. Renewable and Sustainable Energy Reviews, 22, 466–481.

    Article  Google Scholar 

  5. Tollefson, J. (2008). Advanced biofuels face an uncertain future. Nature, 452(7188), 670–671.

    Article  CAS  Google Scholar 

  6. Fathimaa, A. A., Sanithaa, M., Kumarb, T., Iyappana, S., & Ramyaa, M. (2016). Direct utilization of waste water algal biomass for ethanol production by cellulolytic Clostridium phytofermentans DSM1183. Bioresource Technology, 202, 253–256.

    Article  Google Scholar 

  7. Guerriero, G., Hausman, J.-F., Strauss, J., Ertan, H., & Siddiqui, K. S. (2016). Lignocellulosic biomass: biosynthesis, degradation, and industrial utilization. Engineering in Life Sciences, 16(1), 1–16.

    Article  CAS  Google Scholar 

  8. Sindhu, R., Binod, P., & Pandey, A. (2016). Biological pretreatment of lignocellulosic biomass—an overview. Bioresource Technology, 199, 76–82.

    Article  CAS  Google Scholar 

  9. Galbe, M., & Zacchi, G. (2012). Pretreatment: the key to efficient utilization of lignocellulosic materials. Biomass and Bioenergy, 46, 70–78.

    Article  CAS  Google Scholar 

  10. Huang, Y., Qin, X., Luo, X.-M., Nong, Q., Yang, Q., Zhang, Z., Gao, Y., Lv, F., Chen, Y., Yu, Z., Liu, J.-L., & Feng, J.-X. (2015). Efficient enzymatic hydrolysis and simultaneous saccharification and fermentation of sugarcane bagasse pulp for ethanol production by cellulase from Penicillium oxalicum EU2106 and thermotolerant Saccharomyces cerevisiae ZM1-5. Biomass and Bioenergy, 77, 53–63.

    Article  CAS  Google Scholar 

  11. Hilpmann, G., Becher, N., Pahner, F. A., Kusema, B., Mäki-Arvela, P., Lange, R., Murzin, D. Y., & Salmi, T. (2016). Acid hydrolysis of xylan. Catalysis Today, 259, Part 2, 376–380.

    Article  Google Scholar 

  12. Sarkar, N., Ghosh, S. K., Bannerjee, S., & Aikat, K. (2012). Bioethanol production from agricultural wastes: an overview. Renewable Energy, 37(1), 19–27.

    Article  CAS  Google Scholar 

  13. Brodeur, G., Yau, E., Badal, K., Collier, J., Ramachandran, K., & Ramakrishnan, S. (2011). Chemical and physicochemical pretreatment of lignocellulosic biomass: a review. Enzyme Research, 2011, 1–17.

    Article  Google Scholar 

  14. Gao, Z., Zhang, K., Huang, H., Li, S., & Wei, P. (2009). Fumaric acid production by Rhizopus sp. Progress in Chemistry, 251–258.

  15. Liu, H., Ma, J., Wang, M., Wang, W., Deng, L., Nie, K., Yue, X., Wang, F., & Tan, T. (2016). Food waste fermentation to fumaric acid by Rhizopus arrhizus RH7-13. Applied Biochemistry and Biotechnology, 1–10.

  16. Abedinifar, S., Karimi, K., Khanahmadi, M., & Taherzadeh, M. J. (2009). Ethanol production by Mucor indicus and Rhizopus oryzae from rice straw by separate hydrolysis and fermentation. Biomass and Bioenergy, 33(5), 828–833.

    Article  CAS  Google Scholar 

  17. Avelar, E., Jha, R., Beltranena, E., Cervantes, M., Morales, A., & Zijlstra, R. T. (2010). The effect of feeding wheat distillers dried grain with solubles on growth performance and nutrient digestibility in weaned pigs. Animal Feed Science and Technology, 160(1-2), 73–77.

    Article  CAS  Google Scholar 

  18. Liu, H., Yue, X., Jin, Y., Wang, M., Deng, L., Wang, F., & Tan, T. (2017). Preparation of hydrolytic liquid from dried distiller's grains with solubles and fumaric acid fermentation by Rhizopus arrhizus RH 7-13. Journal of Environmental Management, 201, 172–176.

    Article  CAS  Google Scholar 

  19. Liu, H., Hu, H., Jin, Y., Yue, X., Deng, L., Wang, F., & Tan, T. (2017). Co-fermentation of a mixture of glucose and xylose to fumaric acid by Rhizopus arrhizus RH 7-13-9#. Bioresource Technology, 233, 30–33.

    Article  CAS  Google Scholar 

  20. Gu, C., Zhou, Y., Liu, L., Tan, T., & Deng, L. (2013). Production of fumaric acid by immobilized Rhizopus arrhizus on net. Bioresource Technology, 131, 303–307.

    Article  CAS  Google Scholar 

  21. Liu, H., Wang, W., Deng, L., Wang, F., & Tan, T. (2015). High production of fumaric acid from xylose by newly selected strain Rhizopus arrhizus RH 7-13-9#. Bioresource Technology, 186, 348–350.

    Article  CAS  Google Scholar 

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Acknowledgements

This research was financially supported by National Key research program (2016YFD0400601, 2017YFD0400603, 2017YFB0306900), the Natural Science Foundation of China (21476017), the Hong Kong, Macao, and Taiwan scientific and technological cooperation projects (2015DFT30050), the Amoy Industrial Biotechnology R&D and Pilot Conversion Platform (3502Z20121009), and the Fundamental Research Funds for the Central Universities (PYBZ1712).

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Correspondence to Li Deng.

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The authors declared that they have no conflicts of interest.

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Highlights

• Non-pretreated hydrolytic liquid of DDGS could be used as seed culture medium.

• Both glucose and xylose in the hydrolytic liquid were utilized by the strain.

• High yield of bio-ethanol was obtained from non-pretreated hydrolytic liquid.

• This study was significant for the utilization of acid-hydrolytic liquid.

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Liu, H., Zhang, S., Yu, N. et al. Direct Utilization of Non-pretreated Hydrolytic Liquid of Dried Distiller’s Grains with Solubles for Bio-Ethanol by Rhizopus arrhizus RH 7-13-9#. Appl Biochem Biotechnol 186, 590–596 (2018). https://doi.org/10.1007/s12010-018-2716-4

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  • DOI: https://doi.org/10.1007/s12010-018-2716-4

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