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Production of FAME and FAEE via Alcoholysis of Sunflower Oil by Eversa Lipases Immobilized on Hydrophobic Supports

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The performance of two new commercial low-cost lipases Eversa® Transform and Eversa® Transform 2.0 immobilized in different supports was investigated. The two lipases were adsorbed on four different hydrophobic supports. Interesting results were obtained for both lipases and for the four supports. However, the most active derivative was prepared by immobilization of Eversa® Transform 2.0 on Sepabeads C-18. Ninety-nine percent of fatty acid ethyl ester was obtained, in 3 h at 40 °C, by using hexane as solvent, a molar ratio of 4:1 (ethanol/oil), and 10 wt% of immobilized biocatalyst. The final reaction mixture contained traces of monoacylglycerols but was completely free of diacylglycerols. After four reaction cycles, the immobilized biocatalyst preserved 75% of activity. Both lipases immobilized in Sepabeads C-18 were very active with ethanol and methanol as acceptors, but they were much more stable in the presence of ethanol.

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We would like to thank Novozymes and Ramiro Martinez for the generous gift of commercial lipases.

Conflict of Interest

The authors declare that they have no conflict of interest.


This work was sponsored by the Spanish Ministry of Science and Innovation (projects AGL-2009-07526 and BIO2012-36861). The authors thank CNPq and CAPES for the scholarships and financial support of this work.

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Correspondence to Débora de Oliveira.

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Remonatto, D., de Oliveira, J.V., Manuel Guisan, J. et al. Production of FAME and FAEE via Alcoholysis of Sunflower Oil by Eversa Lipases Immobilized on Hydrophobic Supports. Appl Biochem Biotechnol 185, 705–716 (2018).

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