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Structural Characterization and In Vitro Antitumor Activity of a Novel Exopolysaccharide from Lachnum YM130

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Abstract

Exopolysaccharide of Lachnum YM130 (LEP) was purified by diethylaminoethyl cellulose 52 and Sepharose CL-6B column chromatography. LEP-2a was identified to be a homogeneous component with an average molecular weight of 1.31 × 106 Da, which was consisted of mannose and galactose in a molar ratio of 3.8:1.0. The structure of LEP-2a was characterized by methylation analysis, FT-IR analysis, and NMR analysis. Results indicated that LEP-2a was a galactomannan with a backbone, composed of 1,2-linked-α-D-Manp, 1,2,6-linked-α-D-Manp, 1,3,4-linked-α-D-Manp, and 1,3-linked-β-D-Galp, which was substituted at O-2, O-3, O-4, and O-6 by branches. In vitro antitumor activity assay proved that LEP-2a could significantly enhance the inhibitory effectiveness of 5-FU on Hela cells at the concentrations of 100, 200, 300, and 400 μg/mL. The above results suggested that LEP-2a could be seen as a potential source for developing novel antineoplastic agents.

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Funding

The authors would like to acknowledge the support provided by the project “National Natural Science Foundation of China (31470146)” and “Doctoral Research Foundation of HFUT (Grant No.:31700015 JZ2016HGBZ0747).”

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  1. Ping Xu and Ruyue Yuan contributed equally to this work.

Authors and Affiliations

  1. Microbial Resources and Application Laboratory, School of Food Science and Engineering, Hefei University of Technology, Hefei, 230009, China

    Ping Xu, Ruyue Yuan, Guohua Hou, Jinglei Li & Ming Ye

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  1. Ping Xu
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Correspondence to Ming Ye.

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Xu, P., Yuan, R., Hou, G. et al. Structural Characterization and In Vitro Antitumor Activity of a Novel Exopolysaccharide from Lachnum YM130. Appl Biochem Biotechnol 185, 541–554 (2018). https://doi.org/10.1007/s12010-017-2668-0

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