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Bioprocessing of Biodiesel Industry Effluent by Immobilized Bacteria to Produce Value-Added Products

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Abstract

Biodiesel industrial effluent rich in crude glycerol (CG) was processed to produce value-added product. Under continuous culture system, Bacillus amyloliquefaciens strain CD16 immobilized within its biofilm, produced 3.2 L H2/day/L feed, over a period of 60 days at a hydraulic retention time of 2 days. The effective H2 yield by B. amyloliquefaciens strain CD16 was 165 L/L CG. This H2 yield was 1.18-fold higher than that observed with non-biofilm forming Bacillus thuringiensis strain EGU45. Bioprocessing of the effluent released after this stage, by recycling it up to 25% did not have any adverse effect on H2 production by strain EGU45; however, a 25% reduction in yield was recorded with strain CD16. Biofilm forming H2 producers thus proved effective as self-immobilizing system leading to enhanced process efficiency.

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Acknowledgements

We are thankful to the Director of CSIR-Institute of Genomics and Integrative Biology (CSIR-IGIB), and CSIR-HRD (ES Scheme No. 21(1022)/16/EMR-II) for providing the necessary funds, facilities, and moral support. Authors are also thankful to Academy of Scientific & Innovative Research (AcSIR), New Delhi, and JP is also thankful to University Grants Commission (UGC).

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Authors and Affiliations

  1. Microbial Biotechnology and Genomics, CSIR – Institute of Genomics and Integrative Biology (IGIB), Delhi University Campus, Mall Road, New Delhi, 110007, India

    Jyotsana Prakash, Rahul Kumar Gupta, Priyanka XX & Vipin Chandra Kalia

  2. Academy of Scientific and Innovative Research (AcSIR), 2, Rafi Marg, Anusandhan Bhawan, New Delhi, 110001, India

    Jyotsana Prakash, Priyanka XX & Vipin Chandra Kalia

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  1. Jyotsana Prakash
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Prakash, J., Gupta, R.K., XX, P. et al. Bioprocessing of Biodiesel Industry Effluent by Immobilized Bacteria to Produce Value-Added Products. Appl Biochem Biotechnol 185, 179–190 (2018). https://doi.org/10.1007/s12010-017-2637-7

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