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Isolation and Purification of Condensed Tannin from the Leaves and Branches of Prunus cerasifera and Its Structure and Bioactivities

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Abstract

Prunus cerasifera has a rich resource and a weak utilization rate and its biological functions have been investigated. We found that the contents of total phenol (TP) in leaves and branches of Prunus cerasifera were 117.8 ± 8.8 and 100.04 ± 0.9 mg/g, respectively; the contents of soluble condensed tannin (SCT) were 73.95 ± 0.9 and 78.65 ± 4.1 mg/g, respectively; the structure of SCT containing afzelechin/epiafzelechin, catechin/epicatechin, and atechin/epicatechin as the main units and the SCT from leaves and branches exhibited better anti-tyrosinase and antioxidant activities. This study could clarify Prunus cerasifera condensed tannin resource availability and lay a theoretical foundation for its development as a natural antioxidant and tyrosinase inhibitor.

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Abbreviations

SCT:

Soluble condensed tannin

TP:

Total phenol

KI:

Inhibition constant

KIS:

Inhibition constant

HPLC-ESI-MS:

High-performance liquid chromatography electrospray ionization mass spectrometry

DPPH:

2,2-Diphenyl-1-picrylhydrazyl

ABTS:

2,2′-Azinobis(3-ethylbenzothiazoline-6-sulfonic acid)

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Funding

This work was supported by grants from the National Nature Science Foundation of China Grant (Nos. 81402309), Henan Planning Project of Science and Technology (Nos. 172102310331), training scheme for young key teachers of colleges and universities of Henan (Nos. 2016GGJS-139), and Scientific research promotion project of Henan University Of Urban Construction (Nos. 2016QY017).

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Author notes

  1. Wei Song and Shao-Tong Qin contributed equally to this work.

Authors and Affiliations

  1. School of Life Science and Engineering, Henan University of Urban Construction, Pingdingshan, Henan, 467044, China

    Wei Song, Shao-Tong Qin, Fei-Xiang Fang, Zhen-Jiang Gao, Dan-Dan Liang, Lu-Lu Liu, Hong-Tao Tian & Hai-Bo Yang

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  1. Wei Song
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Correspondence to Hai-Bo Yang.

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Song, W., Qin, ST., Fang, FX. et al. Isolation and Purification of Condensed Tannin from the Leaves and Branches of Prunus cerasifera and Its Structure and Bioactivities. Appl Biochem Biotechnol 185, 464–475 (2018). https://doi.org/10.1007/s12010-017-2635-9

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