Applied Biochemistry and Biotechnology

, Volume 184, Issue 2, pp 471–483 | Cite as

Thermal Pretreatment of Harvest Residues and Their Use in Anaerobic Co-digestion with Dairy Cow Manure

  • Đurđica KovačićEmail author
  • Davor Kralik
  • Daria Jovičić
  • Slavko Rupčić
  • Brigita Popović
  • Marina Tišma


Several batch experiments were conducted on the anaerobic co-digestion of dairy cow manure (DCM) with three harvest residues (HR) (soybean straw, sunflower stalks, and corn stover). The influence of thermal pretreatment of HR on biogas production was investigated, where the HR were thermally pretreated at two different temperatures: T = 121 °C and T = 175 °C, during t = 30 and t = 90 min, respectively. All anaerobic co-digestion batch experiments were performed simultaneously under thermophilic regime, at T = 55 °C. Biogas and methane yields were significantly improved in experiments performed with corn stover thermally pretreated at 175 °C for 30 min (491.37 cm3/g VS and 306.96 cm3/g VS, respectively), if compared to experiments performed with untreated corn stover. The highest VS and COD removal rates were also observed in the same group of experiments and were 34.5 and 50.1%, respectively. The highest biogas and methane yields with soybean straw (418.93 cm3/g VS and 261.44 cm3/g VS, respectively) were obtained when soybean straw pretreated at 121 °C during 90 min. The highest biogas and methane yields with sunflower stalk (393.28 cm3/g VS and 245.02 cm3/g VS, respectively) were obtained when sunflower stalk was pretreated at 121 °C during 90 min.


Anaerobic co-digestion Dairy cow manure Soybean straw Sunflower stalks Corn stover 



Anaerobic digestion


Harvest residues


Dairy cow manure


Total solids


Volatile solids


Total organic carbon


Total extractable nitrogen


Chemical oxygen demand



This work was financially supported by project “ProBioTech - Development of innovative process of agricultural waste biological treatment in biogas production” (RC. 2.2.08-0045), which was co-financed by European regional development fund (ERDF).


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Copyright information

© Springer Science+Business Media, LLC 2017

Authors and Affiliations

  1. 1.Faculty of Agriculture in OsijekJ. J. Strossmayer University of OsijekOsijekCroatia
  2. 2.Faculty of Electrical Engineering, Computer Science and Information Technology OsijekJ. J. Strossmayer University of OsijekOsijekCroatia
  3. 3.Faculty of Food Technology OsijekJ. J. Strossmayer University of OsijekOsijekCroatia

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