Applied Biochemistry and Biotechnology

, Volume 184, Issue 1, pp 1–11 | Cite as

Dextran Utilization During Its Synthesis by Weissella cibaria RBA12 Can Be Overcome by Fed-Batch Fermentation in a Bioreactor

  • Rwivoo Baruah
  • Barsha Deka
  • Niharika Kashyap
  • Arun Goyal
Article

Abstract

Weissella cibaria RBA12 produced a maximum of 9 mg/ml dextran (with 90% efficiency) using shake flask culture under the optimized concentration of medium components viz. 2% (w/v) of each sucrose, yeast extract, and K2HPO4 after incubation at optimized conditions of 20 °C and 180 rpm for 24 h. The optimized medium and conditions were used for scale-up of dextran production from Weissella cibaria RBA12 in 2.5-l working volume under batch fermentation in a bioreactor that yielded a maximum of 9.3 mg/ml dextran (with 93% efficiency) at 14 h. After 14 h, dextran produced was utilized by the bacterium till 18 h in its stationary phase under sucrose depleted conditions. Dextran utilization was further studied by fed-batch fermentation using sucrose feed. Dextran on production under fed-batch fermentation in bioreactor gave 35.8 mg/ml after 32 h. In fed-batch mode, there was no decrease in dextran concentration as observed in the batch mode. This showed that the utilization of dextran by Weissella cibaria RBA12 is initiated when there is sucrose depletion and therefore the presence of sucrose can possibly overcome the dextran hydrolysis. This is the first report of utilization of dextran, post-sucrose depletion by Weissella sp. studied in bioreactor.

Keywords

Weissella cibaria Dextran Sucrose Bioreactor 

Notes

Acknowledgement

The authors would like to thank Mr. Katla Srikanth and Dr. Senthilkumar Sivaprakasam of Department of Biosciences and Bioengineering, IIT Guwahati for suggestions and discussion regarding batch and fed-batch processes using bioreactor. The research work was supported by a project grant (BT/PR1518/PID/6/613/2011) from Department of Biotechnology (DBT), New Delhi, India to Dr. A. Goyal. The bioreactor procured through grant was received from Indo-Finland joint project (BT/IN/Finland/08/AG/2011) from Department of Biotechnology, Ministry of Science and Technology, New Delhi, India to Dr. A. Goyal.

Compliance with Ethical Standards

Conflict of Interest

The authors declare that they have no conflict of interest.

References

  1. 1.
    Falconer, D. J., Mukerjea, R., & Robyt, J. F. (2011). Biosynthesis of dextrans with different molecular weights by selecting the concentration of Leuconostoc mesenteroides B-512FMC dextransucrase, the sucrose concentration, and the temperature. Carbohydrate Research, 346(2), 280–284.CrossRefGoogle Scholar
  2. 2.
    Capek, P., Hlavoòová, E., Matulová, M., Mislovicova, D., Rùžièka, J., Koutný, M., & Keprdova, L. (2011). Isolation and characterization of an extracellular glucan produced by Leuconostoc garlicum PR. Carbohydrate Polymers., 83(1), 88–93.CrossRefGoogle Scholar
  3. 3.
    Malang, S. K., Maina, N. H., Schwab, C., Tenkanen, M., & Lacroix, C. (2015). Characterization of exopolysaccharide and ropy capsular polysaccharide formation by Weissella. Food Microbiology, 46, 418–427.CrossRefGoogle Scholar
  4. 4.
    Fusco, V., Quero, G. M., Cho, G. S., Kabisch, J., Meske, D., Neve, H., Bockelmann, W., & Franz, C. M. (2015). The genus Weissella: Taxonomy, ecology and biotechnological potential. Frontiers in Microbiology, 6, 155–177.CrossRefGoogle Scholar
  5. 5.
    Baruah, R., Maina, N. H., Katina, K., Juvonen, R., & Goyal, A. (2017). Functional food applications of dextran from Weissella cibaria RBA12 from pummelo (Citrus maxima). International Journal of Food Microbiology, 242, 124–131.CrossRefGoogle Scholar
  6. 6.
    Ahmed, R. Z., Siddiqui, K., Arman, M., & Ahmed, N. (2012). Characterization of high molecular weight dextran produced by Weissella cibaria CMGDEX3. Carbohydrate Polymers, 90(1), 441–446.CrossRefGoogle Scholar
  7. 7.
    Rao, T. J. M., & Goyal, A. (2013). A novel high dextran yielding Weissella cibaria JAG8 for cereal food application. International Journal of Food Sciences and Nutrition, 64(3), 346–354.CrossRefGoogle Scholar
  8. 8.
    Shukla, S., Shi, Q., Maina, N. H., Juvonen, M., & Goyal, A. (2014). Weissella confusa Cab3 dextransucrase: Properties and in vitro synthesis of dextran and glucooligosaccharides. Carbohydrate Polymers, 101, 554–564.CrossRefGoogle Scholar
  9. 9.
    Tingirikari, J. M. R., Kothari, D., Shukla, R., & Goyal, A. (2014). Structural and biocompatibility properties of dextran from Weissella cibaria JAG8 as food additive. International Journal of Food Sciences and Nutrition, 65(6), 686–691.CrossRefGoogle Scholar
  10. 10.
    Chen, X. Y., & Gänzle, M. G. (2016). Site directed mutagenesis of dextransucrase Dsr M from Weissella cibaria: Transformation to a reuteransucrase. Journal of Agricultural and Food Chemistry, 64(36), 6848–6855.CrossRefGoogle Scholar
  11. 11.
    Li, J. M., & Nie, S. P. (2016). The functional and nutritional aspects of hydrocolloids in foods. Food Hydrocolloids, 53, 46–61.CrossRefGoogle Scholar
  12. 12.
    Kajala, I., Shi, Q., Nyyssölä, A., Maina, N. H., Hou, Y., Katina, K., Tenkanen, M., & Juvonen, R. (2015). Cloning and characterization of a Weissella confusa dextransucrase and its application in high fibre baking. Plo S one, 10(1), e0116418.CrossRefGoogle Scholar
  13. 13.
    Shukla, S., & Goyal, A. (2012). Development of efficient fermentation process at bioreactor level by taguchi’s orthogonal array methodology for enhanced dextransucrase production from Weissella confusa Cab3. Advances in Microbiology, 2(03), 277.CrossRefGoogle Scholar
  14. 14.
    Shukla, S., & Goyal, A. (2011). Optimization of fermentation medium for enhanced glucansucrase and glucan production from Weissella confusa. Brazilian Archives of Biology and Technology, 54(6), 1117–1124.CrossRefGoogle Scholar
  15. 15.
    Goyal, A., Nigam, M., & Katiyar, S. S. (1995). Optimal conditions for production of dextransucrase from Leuconostoc mesenteroides NRLL B-512F and its properties. Journal of Basic Microbiology, 35(6), 375–384.CrossRefGoogle Scholar
  16. 16.
    Santos, M., Rodrigues, A., & Teixeira, J. A. (2005). Production of dextran and fructose from carob pod extract and cheese whey by Leuconostoc mesenteroides NRRL B512 (f). Biochemical Engineering Journal, 25(1), 1–6.CrossRefGoogle Scholar
  17. 17.
    Baruah, R., & Goyal, A. (2015). Hyper glucansucrase, glucan and oligosaccharide producing novel Weissella cibaria RBA12 isolated from Pummelo (Citrus maxima). Annals of Microbiology, 65(4), 2301–2310.CrossRefGoogle Scholar
  18. 18.
    Tsuchiya, H., Koepsell, H., Corman, J., Bryant, G., Bogard, M. O., Feger, V. H., & Jackson, R. W. (1952). The effect of certain cultural factors on production of dextransucrase by Leuconostoc mesenteroides. Journal of Bacteriology, 64, 521.Google Scholar
  19. 19.
    Dubois, M., Gilles, K. A., Hamilton, J. K., Rebers, P., & Smith, F. (1956). Colorimetric method for determination of sugars and related substances. Analytical Chemistry, 28, 350–356.CrossRefGoogle Scholar
  20. 20.
    Miller, G. L. (1959). Use of dinitrosalicylic acid reagent for determination of reducing sugar. Analytical Chemistry, 31(3), 426–428.CrossRefGoogle Scholar
  21. 21.
    Mohan Rao, T. J., & Goyal, A. (2013). Purification, optimization of assay, and stability studies of dextransucrase isolated from Weissella cibaria JAG8. Preparative Biochemistry and Biotechnology, 43(4), 329–341.CrossRefGoogle Scholar
  22. 22.
    Katina, K., Maina, N. H., Juvonen, R., Flander, L., Johansson, L., Virkki, L., et al. (2009). In situ production and analysis of Weissella confusa dextran in wheat sourdough. Food Microbiology, 26(7), 734–743.CrossRefGoogle Scholar
  23. 23.
    Ricciardi, A., Parente, E., & Zotta, T. (2009). Modelling the growth of Weissella cibaria as a function of fermentation conditions. Journal of Applied Microbiology, 107(5), 1528–1535.CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  • Rwivoo Baruah
    • 1
  • Barsha Deka
    • 1
  • Niharika Kashyap
    • 2
  • Arun Goyal
    • 1
    • 2
  1. 1.Carbohydrate Enzyme Biotechnology Laboratory, Department of Bioscience and BioengineeringIndian Institute of Technology GuwahatiGuwahatiIndia
  2. 2.Center for the EnvironmentIndian Institute of Technology GuwahatiGuwahatiIndia

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