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Biosuccinic Acid from Lignocellulosic-Based Hexoses and Pentoses by Actinobacillus succinogenes: Characterization of the Conversion Process

Applied Biochemistry and Biotechnology volume 183, pages 1465–1477 (2017)Cite this article

Abstract

Succinic acid (SA) is a well-established chemical building block. Actinobacillus succinogenes fermentation is by far the most investigated route due to very promising high SA yield and titer on several sugars. This study contributes to include the SA production within the concept of biorefinery of lignocellulose biomass. The study was focused on the SA production by A. succinogenes DSM 22257 using sugars representative from lignocellulose hydrolysis—glucose, mannose, arabinose, and xylose—as carbon source. Single sugar batch fermentation tests and mixture sugar fermentation tests were carried out. All the sugars investigated were converted in succinic acid by A. succinogenes. The best fermentation performances were measured in tests with glucose as carbon source. The bacterial growth kinetics was characterized by glucose inhibition. No inhibition phenomena were observed with the other sugar investigated. The sugar mixture fermentation tests highlighted the synergic effects of the co-presence of the four sugars. Under the operating conditions tested, the final concentration of succinic acid in the sugar mixture test was larger (27 g/L) than that expected (25.5 g/L) by combining the fermentation of the single sugar. Moreover, the concentration of acetic and formic acid was lower, consequently obtaining an increment in the succinic acid specificity.

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Acknowledgments

The study was supported by the Ministero dell’Istruzione, dell’Università e della Ricerca project “Development of green technologies for production of BIOchemicals and their use in preparation and industrial application of POLImeric materials from agricultural biomasses cultivated in a sustainable way in Campania Region—BIOPOLIS” PON03PE_00107_1/1.

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  1. Dipartimento di Ingegneria Chimica, dei Materiali e della Produzione Industriale, Università degli Studi di Napoli Federico II, P.le V. Tecchio 80, 80125, Naples, Italy

    Mariateresa Ferone, Francesca Raganati, Giuseppe Olivieri, Piero Salatino & Antonio Marzocchella

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  1. Mariateresa Ferone
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  2. Francesca Raganati
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  3. Giuseppe Olivieri
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  4. Piero Salatino
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  5. Antonio Marzocchella
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Correspondence to Giuseppe Olivieri.

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Ferone, M., Raganati, F., Olivieri, G. et al. Biosuccinic Acid from Lignocellulosic-Based Hexoses and Pentoses by Actinobacillus succinogenes: Characterization of the Conversion Process. Appl Biochem Biotechnol 183, 1465–1477 (2017). https://doi.org/10.1007/s12010-017-2514-4

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  • DOI: https://doi.org/10.1007/s12010-017-2514-4

Keywords

  • Succinic acid
  • Anaerobic fermentation
  • Actinobacillus succinogenes
  • Lignocellulose
  • Biorefinery