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Identification and High-level Production of Pulcherrimin in Bacillus licheniformis DW2

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Abstract

Pulcherrimin, a potential biocontrol agent produced by microorganisms, has the promising applications in the agricultural, medical, and food areas, and the low yield of pulcherrimin has hindered its applications. In this study, the red pigment produced by Bacillus licheniformis DW2 was identified as pulcherrimin through the spectrometry analysis and genetic manipulation, and the component of the medium used for pulcherrimin production was optimized. Based on our results, the addition of 1.0 g L−1 Tween 80 could improve the yield of pulcherrimin, and glucose and (NH4)2SO4 were served as the optimal carbon and nitrogen sources for pulcherrimin synthesis, respectively. Furthermore, an orthogonal array design was applied for optimization of the medium. Under optimized condition, the maximum yield of pulcherrimin was 331.17 mg L−1, 5.30-fold higher than that of the initial condition, which was the maximum yield reported for pulcherrimin production. Collectively, this study provided a promising strain and a feasible approach to achieve the high-level production of antimicrobial pulcherrimin.

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Acknowledgements

This study was funded by the National Science and Technology Pillar Program during the Twelfth Five-year Plan Period (2013AA102801-52), and the Science and Technology Program of Wuhan (20160201010086).

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Authors and Affiliations

  1. Hubei Collaborative Innovation Center for Green Transformation of Bio-resources, College of Life Sciences, Hubei University, No. 368Youyi Avenue, Wuchang District, Wuhan, 430062, Hubei, People’s Republic of China

    Xiaoyun Li, Dongbo Cai, Yangyang Zhan, Qin Wang & Shouwen Chen

  2. State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, 430070, People’s Republic of China

    Dong Wang & Shouwen Chen

Authors
  1. Xiaoyun Li
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  2. Dong Wang
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  3. Dongbo Cai
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  4. Yangyang Zhan
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  5. Qin Wang
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  6. Shouwen Chen
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Contributions

D. Wang and S. Chen designed and supervised the study. X. Li performed the experiments. X. Li, D. Wang, D. Cai, Y. Zhan, Q. Wang, and S. Chen analyzed the data and wrote the manuscript. All authors read and approved the final manuscript.

Corresponding author

Correspondence to Shouwen Chen.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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The authors declare that they have no conflict of interest.

Electronic supplementary material

All primers used in this study and the result about effects of K2HPO4·3H2O, MgSO4·7H2O, CaCl2·H2O, FeCl3·6H2O, and MnSO4·H2O on pulcherrimin production were listed as the supplementary materials. This information is available free of charge via https://link.springer.com/journal/12010.

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Li, X., Wang, D., Cai, D. et al. Identification and High-level Production of Pulcherrimin in Bacillus licheniformis DW2. Appl Biochem Biotechnol 183, 1323–1335 (2017). https://doi.org/10.1007/s12010-017-2500-x

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  • DOI: https://doi.org/10.1007/s12010-017-2500-x

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