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Metal-Chelate Affinity Precipitation with Thermo-Responsive Polymer for Purification of ε-Poly-l-Lysine

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Abstract

ε-Poly-l-lysine (ε-PL) is a natural preservative for food processing industry. A thermo-responsive polymer, attached with Cu2+ or Ni2+, was prepared for metal-chelate affinity precipitation for purification of ε-PL. The low critical solution temperatures (LCSTs) of these polymers were close to the room temperature (31.0–35.0 °C). The optimal adsorption conditions were as follows: pH 4.0, 0 mol/L NaCl, ligand density 75.00 μmol/g, and 120 min. The ligand Cu2+ showed a stronger affinity interaction with ε-PL and the highest adsorption amount reached 251.93 mg/g polymer. The elution recovery of ε-PL could be 98.42% with 0.50 mol/L imidazole (pH = 8.0) as the eluent. The method could purify ε-PL from fermentation broth and the final product was proved as electrophoretic pure by SDS-PAGE. Moreover, these affinity polymers could be recycled after the purification of ε-PL and the recoveries were above 95.00%.

Scheme for affinity precipitation of ε-PL

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Acknowledgements

This work was supported by the National Special Fund for State Key Laboratory of Bioreactor Engineering (No. 2060204) in East China University of Science and Technology. The technical support from instrument platform in State Key Laboratory of Bioreactor Engineering also was highly appreciated.

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Authors and Affiliations

  1. State Key Laboratory of Bioreactor Engineering, Department of Bioengineering, East China University of Science and Technology, Shanghai, 200237, China

    Sipeng Li, Zhaoyang Ding, Jifu Liu & Xuejun Cao

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  1. Sipeng Li
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  2. Zhaoyang Ding
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  3. Jifu Liu
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Correspondence to Xuejun Cao.

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Li, S., Ding, Z., Liu, J. et al. Metal-Chelate Affinity Precipitation with Thermo-Responsive Polymer for Purification of ε-Poly-l-Lysine. Appl Biochem Biotechnol 183, 1254–1264 (2017). https://doi.org/10.1007/s12010-017-2495-3

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  • DOI: https://doi.org/10.1007/s12010-017-2495-3

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