Skip to main content

Model-Based Nutrient Feeding Strategies for the Increased Production of Polyhydroxybutyrate (PHB) by Alcaligenes latus

Abstract

Polyhydroxyalkanoates (PHAs) are biodegradable polymers which are considered as an effective alternative for conventional plastics due to their mechanical properties similar to the latter. However, the widespread use of these polymers is still hampered due to their higher cost of production as compared to plastics. The production cost could be overcome by obtaining high yields and productivity. The goal of the present research was to enhance the yield of polyhydroxybutyrate (PHB) with the help of two simple fed-batch cultivation strategies. In the present study, average batch kinetic and substrate limitation/inhibition study data of Alcaligenes latus was used for the development of PHB model which was then adopted for designing various off-line nutrient feeding strategies to enhance PHB accumulation. The predictive ability of the model was validated by experimental implementation of two fed-batch strategies. One such dynamic strategy of fed-batch cultivation under pseudo-steady state with respect to nitrogen and simultaneous carbon feeding strategy resulted in significantly high biomass and PHB concentration of 39.17 g/L and 29.64 g/L, respectively. This feeding strategy demonstrated a high PHB productivity and PHB content of 0.6 g/L h and 75%, respectively, which were remarkably high in comparison to batch cultivation. The mathematical model can also be employed for designing various other nutrient feeding strategies.

This is a preview of subscription content, access via your institution.

Fig. 1
Fig. 2
Fig. 3

References

  1. Mozumder, M. S. I., Garcia-Gonzalez, L., De Wever, H., & Volcke, E. I. (2016). Model-based process analysis of heterotrophic-autotrophic poly (3-hydroxybutyrate)(PHB) production. Biochemical Engineering Journal, 114, 202–208.

    CAS  Article  Google Scholar 

  2. Novak, M., Koller, M., Braunegg, M., & Horvat, P. (2015). Mathematical modelling as a tool for optimized PHA production. Chemical and Biochemical Engineering Quarterly, 29, 183–220.

    CAS  Article  Google Scholar 

  3. Chanprateep, S. (2010). Current trends in biodegradable polyhydroxyalkanoates. Journal of Bioscience and Bioengineering, 110, 621–632.

    CAS  Article  Google Scholar 

  4. Hafuka, A., Sakaida, K., Satoh, H., Takahashi, M., Watanabe, Y., & Okabe, S. (2011). Effect of feeding regimens on polyhydroxybutyrate production from food wastes by Cupriavidus necator. Bioresource Technology, 102, 3551–3553.

    CAS  Article  Google Scholar 

  5. Shen, L., Haufe, J., & Patel, M. K. (2009). Product overview and market projection of emerging bio-based plastics PRO-BIP 2009. Report for European Polysaccharide Network of Excellence (EPNOE) and European Bioplastics, 243.

  6. Kaur, G., & Roy, I. (2015). Strategies for large-scale production of polyhydroxyalkanoates. Chemical and Biochemical Engineering Quarterly, 29, 157–172.

    CAS  Article  Google Scholar 

  7. Gahlawat, G., & Srivastava, A. K. (2013). Development of a mathematical model for the growth associated Polyhydroxybutyrate fermentation by Azohydromonas australica and its use for the design of fed-batch cultivation strategies. Bioresource Technology, 137, 98–105.

    CAS  Article  Google Scholar 

  8. Dixit, P., Mehta, A., Gahlawat, G., Prasad, G. S., & Choudhury, A. R. (2015). Understanding the effect of interaction among aeration, agitation and impeller positions on mass transfer during pullulan fermentation by Aureobasidium pullulans. RSC Advances, 5(49), 38984–38994.

    CAS  Article  Google Scholar 

  9. Khanna, S., & Srivastava, A. K. (2006a). Computer simulated fed-batch cultivation for over production of PHB: a comparison of simultaneous and alternate feeding of carbon and nitrogen. Biochemical Engineering Journal, 27, 197–203.

    CAS  Article  Google Scholar 

  10. Zinn, M., Weilenmann, H. U., Hany, R., Schmid, M., & Egli, T. (2003). Tailored synthesis of poly ([R]-3-hydroxybutyrate-co-3-hydroxyvalerate)(PHB/HV) in Ralstonia eutropha DSM 428. Acta Biotechnologica, 23, 309–316.

    CAS  Article  Google Scholar 

  11. Mozumder, M. S. I., De Wever, H., Volcke, E. I., & Garcia-Gonzalez, L. (2014). A robust fed-batch feeding strategy independent of the carbon source for optimal polyhydroxybutyrate production. Process Biochemistry, 49, 365–373.

    CAS  Article  Google Scholar 

  12. Khanna, S., & Srivastava, A. K. (2006b). Optimization of nutrient feed concentration and addition time for production of poly (β-hydroxybutyrate). Enzyme Microbial Technology, 39(5), 1145–1151.

    CAS  Article  Google Scholar 

  13. Patwardhan, P., & Srivastava, A. K. (2008). Fed-batch cultivation of Wautersia eutropha. Bioresource Technology, 99, 1787–1792.

    CAS  Article  Google Scholar 

  14. Gahlawat, G., Sengupta, B., & Srivastava, A. K. (2012). Enhanced production of poly(3-hydroxybutyrate) in a novel airlift reactor with in situ cell retention using Azohydromonas australica. Journal of Industrial Microbiology Biotechnology, 39, 1377–1384.

    CAS  Article  Google Scholar 

  15. Gahlawat, G., & Srivastava, A. K. (2012). Estimation of fundamental kinetic parameters of polyhydroxybutyrate fermentation process of Azohydromonas australica using statistical approach of media optimization. Applied Biochemistry and Biotechnology, 168, 1051–1064.

    CAS  Article  Google Scholar 

  16. Volesky, B., & Votruba, J. (1992). Mathematical model identification. In Modeling and optimization of fermentation process (pp. 38–54). Amsterdam: Elsevier.

    Google Scholar 

  17. Rosenbrock, H. H. (1960). An automatic method of finding the greatest or the least value of a function. Computer Journal, 3, 175–184.

    Article  Google Scholar 

  18. Kaur, G., Srivastava, A. K., & Chand, S. (2012). Mathematical modeling approach for concentration and productivity enhancement of 1,3-propanediol using Clostridium diolis. Biochemical Engineering Journal, 68, 34–41.

    CAS  Article  Google Scholar 

  19. Miller, G. L. (1959). Use of dinitrosalicylic acid reagent for determination of reducing sugar. Analytical Chemistry, 31, 426–428.

    CAS  Article  Google Scholar 

  20. Horwitz, W. (1980). Official methods of analysis of the Association of Official Analytical Chemist (thirteenth ed.). Washington, DC: AOAC Methods.

    Google Scholar 

  21. Riis, V., & Mai, W. (1988). Gas chromatographic determination of polyβ-hydroxybutyric acid in microbial biomass after hydrochloric acid propanolysis. Journal of Chromatography A, 445, 285–289.

    CAS  Article  Google Scholar 

  22. Grothe, E., Moo-Young, M., & Chisti, Y. (1999). Fermentation optimization for the production of poly(β-hydroxybutyric acid) microbial thermoplastic. Enzyme and Microbial Technology, 25, 132–141.

    CAS  Article  Google Scholar 

  23. Grothe, E., & Chisti, Y. (2000). Poly (β-hydroxybutyric acid) thermoplastic production by Alcaligenes latus: behavior of fed-batch cultures. Bioprocess Engineering, 22, 441–449.

    CAS  Article  Google Scholar 

  24. Loo, C. Y., & Sudesh, K. (2007). Polyhydroxyalkanoates: bio-based microbial plastics and their properties. Malaysian Polymer Journal, 2, 31–57.

    Google Scholar 

  25. Zafar, M., Kumar, S., Kumar, S., & Dhiman, A. K. (2012). Artificial intelligence based modeling and optimization of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) production process by using Azohydromonas lata MTCC 2311 from cane molasses supplemented with volatile fatty acids: a genetic algorithm paradigm. Bioresource Technology, 104, 631–641.

    CAS  Article  Google Scholar 

  26. Penloglou, G., Chatzidoukas, C., & Kiparissides, C. (2012). Microbial production of polyhydroxybutyrate with tailor-made properties: an integrated modelling approach and experimental validation. Biotechnology Advances, 30, 329–337.

    CAS  Article  Google Scholar 

  27. Chatzidoukas, C., Penloglou, G., & Kiparissides, C. (2013). Development of a structured dynamic model for the production of polyhydroxybutyrate (PHB) in Azohydromonas lata cultures. Biochemical Engineering Journal, 71, 2–80.

    Article  Google Scholar 

  28. Yu, P. H., Chua, H., Huang, A. L., & Ho, K. P. (1999). Conversion of industrial food wastes by Alcaligenes latus into polyhydroxyalkanoates. Applied Biochemistry and Biotechnology, 78, 445–454.

    Article  Google Scholar 

  29. Cavalheiro, J. M. B. T., de Almeida, M. C. M. D., Grandfils, C., & da Fonseca, M. M. R. (2009). Poly(3-hydroxybutyrate) production by Cupriavidus necator using waste glycerol. Process Biochemistry, 44, 509–515.

    CAS  Article  Google Scholar 

  30. Ienczak, J., Quines, L., Melo, A. D., Brandellero, M., Mendes, C., Schmidell, W., & Aragão, G. (2011). High cell density strategy for poly (3-hydroxybutyrate) production by Cupriavidus necator. Brazilian Journal of Chemical Engineering, 28, 585–596.

    CAS  Article  Google Scholar 

  31. Sayed, E. I., Azhar, A., Abdelhady, H. M., Abdel Hafez, A. M., & Khodair, T. A. (2009). Batch production of polyhydroxybutyrate (PHB) by Ralstonia eutropha and Alcaligenes latus using bioreactor different culture strategies. Journal of Applied Sciences Research, 5, 556–564.

    Google Scholar 

  32. Penloglou, G., Roussos, A., Chatzidoukas, C., & Kiparissides, C. (2010). A combined metabolic/polymerization kinetic model on the microbial production of poly (3-hydroxybutyrate). New Biotechnology, 27, 358–367.

    CAS  Article  Google Scholar 

Download references

Acknowledgements

The Senior Research Fellowship (SRF) award by the Department of Biotechnology (DBT), Govt. of India, New Delhi, for the execution of the project is gratefully acknowledged by one of the authors (Ms. Geeta Gahlawat).

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Ashok K. Srivastava.

Rights and permissions

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Gahlawat, G., Srivastava, A.K. Model-Based Nutrient Feeding Strategies for the Increased Production of Polyhydroxybutyrate (PHB) by Alcaligenes latus . Appl Biochem Biotechnol 183, 530–542 (2017). https://doi.org/10.1007/s12010-017-2482-8

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12010-017-2482-8

Keywords

  • Polyhydroxybutyrate
  • Alcaligenes latus
  • Kinetic modeling
  • Nutrient feeding
  • Pseudo-steady state