Enhancing Menaquinone-7 Production by Bacillus natto R127 Through the Nutritional Factors and Surfactant
- 395 Downloads
Bacillus natto is commonly used in industrial production of menaquinone-7, an important vitamin which plays a crucial role for blood clotting and may contribute to prevention of cardiovascular disease and osteoporosis. This study determined the optimal combination of key nutrients and established an effective use of surfactant in a coupling medium to enhance the yield of extracellular MK-7. MK-7 yield of 31.18 mg/L was achieved under optimal conditions containing 53.6 g/L glycerol, 100 g/L soy peptone, and 10 g/L K2HPO4. A maximal yield of 40.96 mg/L MK-7 and a secretion ratio of 61.1% were obtained when 20 g/L soybean oil was supplemented at the logarithmic phase. The non-ionic surfactant span 20 was the second most promising surfactant in improving product yield, whereas addition of 2 g/L betaine exerted a minimal effect on secretion ratio of MK-7 at 19.1%. The results collectively showed that the supplementation of surfactants was an effective strategy to regulate cytomembrane permeability.
KeywordsBacillus natto Menaquinone-7 Optimization Surfactant Secretion ratio
Compliance with Ethical Standards
This work was financially supported by the National Science Foundation for Distinguished Young Scholars of China (No. 21225626), the National Natural Science Foundation of China (No. 21306085 and No. 21476111), Jiangsu Province Outstanding Youth Fund (BK20160092), the National High Technology Research and Development Program of China (No.2014AA021701), the Specialized Research Fund for the Doctoral Program of Higher Education (No. 20133221120008), and the selected project of Nanjing Tech University (No. ZKRC201510).
- 4.Gast, G. C. M., de Roos, N. M., Sluijs, I., Bots, M. L., Beulens, J. W. J., Geleijnse, J. M., Witteman, J. C., Grobbee, D. E., Peeters, P. H. M., & van der Schouw, Y. T. (2009). A high menaquinone intake reduces the incidence of coronary heart disease. Nutrition Metabolism and Cardiovascular Diseases, 19, 504–510.CrossRefGoogle Scholar
- 5.Vos, M., Esposito, G., Edirisinghe, J. N., Vilain, S., Haddad, D. M., Slabbaert, J. R., Meensel, S. V., Schaap, O., Strooper, B. D., Meganathan, R., Morais, V. A., & Verstreken, P. (2012). Vitamin K2 is a mitochondrial electron carrier that rescues pink1 deficiency. Science, 336, 1306–1310.CrossRefGoogle Scholar
- 8.Sandhu, S. K., Oberoi, H. S., Babbar, N., Miglani, K., Chadha, B. S., & Nanda, D. K. (2013). Two-stage statistical medium optimization for augmented cellulase production via solid-state fermentation by newly isolated Aspergillus niger HN-1 and application of crude cellulase consortium in hydrolysis of rice straw. Journal of Agricultural and Food Chemistry, 61, 12653–12661.CrossRefGoogle Scholar
- 9.Pan, X. J., Kadla, J. F., Ehara, K., Gilkes, N., & Saddler, J. N. (2006). Organosolv ethanol lignin from hybrid poplar as a radical scavenger: relationship between lignin structure, extraction conditions, and antioxidant activity. Journal of Agricultural and Food Chemistry, 54, 5806–5813.CrossRefGoogle Scholar
- 15.Berenjian, A., Mahanama, R., Talbot, A., Regtop, H., Kavanagh, J., & Dehghani, F. (2014). Designing of an intensification process for biosynthesis and recovery of menaquinone-7. Applied Microbiology and Biotechnology, 172, 1347–1357.Google Scholar
- 25.Berenjian, A. (2013). Development of new approaches for liquid state fermentation of menaquinone-7. PhD thesis. University of Sydney, Sydney, Australia.Google Scholar
- 26.Zhang, Z. B., Zeng, G. M., Shi, J. G., Liu, J., & Yang, W. C. (2006). Effect of tween-80 and rhamnolipid on the production of protease from Pseudomonas Aeruginosa and Bacillus Subtilis. Acta Scientiae Circumstantiae, 26, 1152–1158.Google Scholar