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Trypsin Slows the Aging of Mice due to Its Novel Superoxide Scavenging Activity

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Abstract

Trypsin is an endogenous enzyme that is generally used as a proteinase. Intriguingly, we found that trypsin had superoxide scavenging activity. In the current study, our results showed that trypsin scavenges superoxide in either intracorporal or extracorporal systems. In the light of the porcupine plots of trypsin compounds generated by ProDy, the copper ion binds to trypsin and accelerates the superoxide scavenging activity of trypsin by increasing the stability of the structure. Furthermore, the data on the age-related parameters showed that the aging of mice could be slowed by trypsin, at least in part, due to its superoxide scavenging activity. These results suggested that trypsin is an effective superoxide scavenger and has potential as a novel agent to promote health and improve aging-associated pathologies.

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Abbreviations

AD:

Alzheimer’s disease

AR/HRP:

Amplex Red/horseradish peroxidase

DDC:

Diethyldithiocarbamate

d-gal:

d-Galactose

DTPA:

Diethylenetriaminepentaacetic acid

ESR:

Electron spin resonance

LF:

Lipofuscin

MAO:

Monoamine oxidase

MDA:

Malondialdehyde

PD:

Parkinson’s disease

ROS:

Reactive oxygen species

SOD:

Superoxide dismutase

STI:

Soybean trypsin inhibitor

Tiron:

1,2-Dihydroxybenzene-3,5-disulfonic acid

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Acknowledgments

We wish to thank Prof. James A. Imlay for providing strains of wild-type and sod knockout mutant for this work. Aging model of mice was established by Western Biotechnology of China. Determination of superoxide anion by ESR was accomplished with support from the Instrumental Analysis and Research Center, Lanzhou University. And we also thank the English editing by ACS ChemWorx (Certificate Verification Key: CBAE-B95E-C9A7-7B41-4C42).

Authors Contribution

X. Li, C.Y. Zhao, and Y.H. Liu conceived the projects. X. Li and X.Y. Pang designed and executed the experiments. Z.C. Tang, Y.H. Liu, and Z.C. Tang contributed expertise in aging of mice and Escherichia coli system. Z.C. Tang, C.Y. Zhao, and X.L. Li analyzed the data. All authors discussed the results. X. Li and C.Y. Zhao co-wrote the paper.

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Authors and Affiliations

  1. College of Food and Bioengineering, Henan University of Science and Technology, Luoyang, 471023, China

    Xin Li, Zichen Tang, Xinling Li & Yunhong Liu

  2. Luoyang Engineering and Technology Research Center of Microbial Fermentation, No. 263 Kaiyuan Avenue, Luolong District, Luoyang City, Henan Province, 471023, China

    Xin Li & Yunhong Liu

  3. College of Medical Technology and Engineering, Henan University of Science and Technology, Luoyang, 471003, China

    Xinyue Pang

  4. School of Pharmacy, Lanzhou University, Lanzhou, 730000, China

    Chunyan Zhao

Authors
  1. Xin Li
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  2. Zichen Tang
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  3. Xinyue Pang
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  5. Xinling Li
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Corresponding author

Correspondence to Xin Li.

Ethics declarations

The experimental procedures were approved by the Animal Test Center of Henan Science and Technology University, license number SCXK (Yu) 2005-0001.

Conflict of Interest

The authors declare that they have no conflict of interest.

Funding Sources

This work was supported by the National Natural Science Foundation of China (Nos. 31000017 and U1404334) and the State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences (KF2015-17).

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Li, X., Tang, Z., Pang, X. et al. Trypsin Slows the Aging of Mice due to Its Novel Superoxide Scavenging Activity. Appl Biochem Biotechnol 181, 1549–1560 (2017). https://doi.org/10.1007/s12010-016-2301-7

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  • DOI: https://doi.org/10.1007/s12010-016-2301-7

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