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Recovery of Phenolic Acid and Enzyme Production from Corn Silage Biologically Treated by Trametes versicolor

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Abstract

Corn silage is used as high-energy forage for dairy cows and more recently for biogas production in a process of anaerobic co-digestion with cow manure. In this work, fresh corn silage after the harvest was used as a substrate in solid-state fermentations with T. versicolor with the aim of phenolic acid recovery and enzyme (laccase and manganese peroxidase) production. During 20 days of fermentation, 10.4-, 3.4-, 3.0-, and 1.8-fold increments in extraction yield of syringic acid, vanillic acid, p-hydroxybenzoic acid, and caffeic acid, respectively, were reached when compared to biologically untreated corn silage. Maximal laccase activity was gained on the 4th day of fermentation (V.A. = 180.2 U/dm3), and manganese peroxidase activity was obtained after the 3rd day of fermentation (V.A. = 30.1 U/dm3). The addition of copper(II) sulfate as inducer during solid state fermentation resulted in 8.5- and 7-fold enhancement of laccase and manganese peroxidase activities, respectively. Furthermore, the influence of pH and temperature on enzyme activities was investigated. Maximal activity of laccase was obtained at T = 50 °C and pH = 3.0, while manganese peroxidase is active at temperature range T = 45–70 °C with the maximal activity at pH = 4.5.

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Acknowledgment

The authors acknowledge financial support from the ProBioTech project (RC. 2.2.08-0045) which is co-financed by the European Regional Development Fund (ERDF).

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Authors and Affiliations

  1. Faculty of Food Technology Osijek, Josip Juraj Strossmayer University of Osijek, Franje Kuhača 20, HR-31000, Osijek, Croatia

    Ana Bucić-Kojić, Gordana Šelo, Mirela Planinić & Marina Tišma

  2. Faculty of Chemical Engineering and Technology, University of Zagreb, Marulićev trg 19, HR-10000, Zagreb, Croatia

    Bruno Zelić

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  1. Ana Bucić-Kojić
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  2. Gordana Šelo
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Correspondence to Marina Tišma.

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Bucić-Kojić, A., Šelo, G., Zelić, B. et al. Recovery of Phenolic Acid and Enzyme Production from Corn Silage Biologically Treated by Trametes versicolor . Appl Biochem Biotechnol 181, 948–960 (2017). https://doi.org/10.1007/s12010-016-2261-y

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