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Improvement and Characterization in Enzymatic Hydrolysis of Regenerated Wheat Straw Dissolved by LiCl/DMAc Solvent System

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Abstract

Lithium chloride (LiCl)/N,N-dimethylacetamide (DMAc) solvent system was used to dissolve native and pretreated wheat straw materials in order to promote the enzymatic hydrolysis process. The dissolution ratio of wheat straw in LiCl/DMAc solvent system increased when dilute sulfuric acid or ethanol-sulfuric acid mixture pretreatment was conducted before dissolution. The materials regenerated from LiCl/DMAc solution exhibited obvious changes in structure and morphology, as revealed by scanning electron microscopy (SEM) and X-ray diffraction (XRD) analysis. The cellulose and xylan digestibilities of regenerated materials were improved obviously. The cellulose digestibilities of materials regenerated from native and pretreated wheat straw by dilute sulfuric acid and ethanol-sulfuric acid mixture were 61.8, 81.2, and 84.4 %, respectively, with 25 FPU of cellulase and 187 IU of xylanase after 96 h. These values were significantly higher than the digestibilities of 0, 33.2, and 57.5 % obtained from corresponding materials before dissolution. Dissolution treatment of wheat straw by LiCl/DMAc solvent system provides an alternative method for efficient enzymatic hydrolysis.

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Acknowledgments

The authors gratefully acknowledge the Science and Technology Project of Huaian (HAS2015035), the financial support from the Project of Jiangsu Province Science and Technology (BE2013083, BE2014101, BE2016706), Natural Science Foundation of Guangdong Province, China (2016A030310124), the Project of National Natural Science Foundation of China (51303181, 51508547), project of Guangzhou Science and Technology (201610010014), Youth Innovation Promotion Association CAS (2015290), the Science and Technology Planning Project of Guangdong Province, China (2016A010104009, 2016A010105016), and the Foundation of Director of Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences (y407r41001). We thank Dr. M. Asraful Alam at GIEC-CAS for helpful discussions and language editing.

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Authors and Affiliations

  1. Key Laboratory of Renewable Energy, Chinese Academy of Sciences, No.2 Nengyuan Road, Tianhe District, Guangzhou, 510640, People’s Republic of China

    Gaoxiang Qi, Lian Xiong, Bo Wang, Xiaoqing Lin, Hairong Zhang, Hailong Li, Chao Huang, Xuefang Chen, Can Wang & Xinde Chen

  2. Guangdong Key Laboratory of New and Renewable Energy Research and Development, Guangzhou, 510640, People’s Republic of China

    Gaoxiang Qi, Lian Xiong, Bo Wang, Xiaoqing Lin, Hairong Zhang, Hailong Li, Chao Huang, Xuefang Chen, Can Wang & Xinde Chen

  3. Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, Guangzhou, 510640, People’s Republic of China

    Gaoxiang Qi, Lian Xiong, Bo Wang, Xiaoqing Lin, Hairong Zhang, Hailong Li, Chao Huang, Xuefang Chen, Can Wang & Xinde Chen

  4. Xuyi Center of Attapulgite Applied Technology Research Development and Industrialization, Chinese Academy of Sciences, Xuyi, 211700, People’s Republic of China

    Gaoxiang Qi, Lian Xiong, Bo Wang, Xiaoqing Lin, Hairong Zhang, Hailong Li, Chao Huang, Xuefang Chen, Can Wang & Xinde Chen

  5. University of Chinese Academy of Sciences, Beijing, 100049, People’s Republic of China

    Gaoxiang Qi & Bo Wang

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  1. Gaoxiang Qi
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Correspondence to Xinde Chen.

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Qi, G., Xiong, L., Wang, B. et al. Improvement and Characterization in Enzymatic Hydrolysis of Regenerated Wheat Straw Dissolved by LiCl/DMAc Solvent System. Appl Biochem Biotechnol 181, 177–191 (2017). https://doi.org/10.1007/s12010-016-2206-5

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