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Effects of Melatonin on Colchicine-Treated PLBs of Dendrobium sonia-28 Orchid

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Abstract

Dendrobium hybrid orchid is popular in orchid commercial industry due to its short life cycle and ability to produce various types of flower colours. This study was conducted to identify the morphological, biochemical and scanning electron microscopy (SEM) analysis in the Dendrobium sonia-28 orchid plants. In this study, 0.05 and 0.075 % of colchicine-treated Dendrobium sonia-28 (4-week-old culture) protocorm-like bodies (PLBs) were treated in different concentrations of melatonin (MEL) posttreatments (0, 0.05, 0.1, 0.5, 1, 5 and 10 μM). Morphological parameters such as number of shoots, growth index and number of PLBs were determined. In the 0.05 and 0.075 % of colchicine-treated PLBs which were posttreated with 0.05 μM MEL resulted in the highest value of the morphological parameters tested based on the number of shoots (84.5 and 96.67), growth index (16.94 and 12.15) and number of PLBs (126.5 and 162.33), respectively. SEM analysis of the 0.05 μM MEL posttreatment on both the colchicine-treated regenerated PLBs showed irregular cell lineages, and some damages occurred on the stomata. This condition might be due to the effect of plasmolyzing occurred in the cell causing irregular cell lineages.

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Abbreviations

MEL:

Melatonin

SEM:

Scanning electron microscopy

PLBs:

Protocorm-like bodies

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Acknowledgments

The authors are grateful to USM Research University Grant (1001/PBIOLOGI/811309) for funding this project.

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Authors and Affiliations

  1. School of Biological Sciences, Universiti Sains Malaysia, Georgetown, 11800, Penang, Malaysia

    M . S. Lim, J. J. J. Antony, Z . Suhana & S. Sreeramanan

  2. Plant Genetic Engineering Laboratory, Institute of Biological Sciences, University of Rajshahi, Rajshahi, Bangladesh

    S. M. Shahinul Islam

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  1. M . S. Lim
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  2. J. J. J. Antony
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  3. S. M. Shahinul Islam
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  4. Z . Suhana
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Correspondence to S. Sreeramanan.

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Lim, M...S., Antony, J.J.J., Islam, S.M.S. et al. Effects of Melatonin on Colchicine-Treated PLBs of Dendrobium sonia-28 Orchid. Appl Biochem Biotechnol 181, 15–31 (2017). https://doi.org/10.1007/s12010-016-2196-3

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  • DOI: https://doi.org/10.1007/s12010-016-2196-3

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