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Detoxification of Olive Mill Wastewater and Bioconversion of Olive Crop Residues into High-Value-Added Biomass by the Choice Edible Mushroom Hericium erinaceus

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Abstract

Environmentally acceptable disposal of olive cultivation residues (e.g., olive prunings; olive pruning residues (OLPR)) and olive mill wastes is of paramount importance since they are generated in huge quantities within a short time. Moreover, olive mill wastewater (OMW) or sludge-like effluents (“alperujo”; two-phase olive mill waste (TPOMW)) are highly biotoxic. Hericium erinaceus is a white-rot fungus which produces choice edible mushrooms on substrates rich in lignocellulosics, and its suitability for the treatment of olive by-products was examined for the first time. Fungal growth resulted in a notable reduction of OMW’s pollution parameters (i.e., 65 % decolorization, 47 % total phenolic reduction, and 52 % phytotoxicity decrease) and correlated with laccase and manganese peroxidase activities. Solid-state fermentation of various mixtures of OLPR, TPOMW, and beech sawdust (control) by H. erinaceus qualified OLPR in subsequent cultivation experiments, where it exhibited high mushroom yields and biological efficiency (31 %). Analyses of proximate composition and bioactive compound content revealed that mushrooms deriving from OLPR substrates showed significantly higher crude fat, total glucan, β-glucan, total phenolics, and ferric-reducing antioxidant potential values than the control. H. erinaceus demonstrated the potential to detoxify OMW and bioconvert OLPR into high-quality biomass, and hence, this fungus could be successfully exploited for the treatment of such by-products.

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Acknowledgments

This research has been co-financed by European Union (European Social Fund (ESF)) and Greek national funds through the Operational Program “Education and Lifelong Learning” of the National Strategic Reference Framework (NSRF)-Research Funding Program entitled “Metagenomics of ligninolytic microorganisms—bioconversion of plant by-products into high-added value products” (THALIS-UOA-MIS 377062).

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Authors and Affiliations

  1. Laboratory of General and Agricultural Microbiology, Agricultural University of Athens, Iera Odos 75, 11855, Athens, Greece

    Georgios Koutrotsios, Evangelia Larou & Georgios I. Zervakis

  2. Department of Nutritional Physiology and Feeding, Agricultural University of Athens, Iera Odos 75, 11855, Athens, Greece

    Konstantinos C. Mountzouris

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  1. Georgios Koutrotsios
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  2. Evangelia Larou
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  3. Konstantinos C. Mountzouris
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  4. Georgios I. Zervakis
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Correspondence to Georgios I. Zervakis.

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Koutrotsios, G., Larou, E., Mountzouris, K.C. et al. Detoxification of Olive Mill Wastewater and Bioconversion of Olive Crop Residues into High-Value-Added Biomass by the Choice Edible Mushroom Hericium erinaceus . Appl Biochem Biotechnol 180, 195–209 (2016). https://doi.org/10.1007/s12010-016-2093-9

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