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Microbial Degradation of Chlorogenic Acid by a Sphingomonas sp. Strain

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Abstract

In order to elucidate the metabolism of chlorogenic acid by environmental microbes, a strain of Sphingomonas sp. isolated from tobacco leaves was cultured under various conditions, and chlorogenic acid degradation and its metabolites were investigated. The strain converting chlorogenic acid was newly isolated and identified as a Sphingomonas sp. strain by 16S rRNA sequencing. The optimal conditions for growth and chlorogenic acid degradation were 37 °C and pH 7.0 with supplementation of 1.5 g/l (NH4)2SO4 as the nitrogen source and 2 g/l chlorogenic acid as the sole carbon source. The maximum chlorogenic acid tolerating capability for the strain was 5 g/l. The main metabolites were identified as caffeic acid, shikimic acid, and 3,4-dihydroxybenzoic acid based on gas chromatography-mass spectrometry analysis. The analysis reveals the biotransformation mechanism of chlorogenic acid in microbial cells isolated from the environment.

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Acknowledgments

This work was supported in part by grants from the Chinese National Science Foundation for Excellent Young Scholars (31422004), the Chinese National Natural Science Foundation (31270154).

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    Authors and Affiliations

    1. China Tobacco Henan Industrial Co. Ltd, Henan, Zhengzhou, 450000, People’s Republic of China

      Yuping Ma, Zhan Zhang, Zongcan Yang & Cong Nie

    2. State Key Laboratory of Microbial Metabolism, and School of Life Sciences & Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, People’s Republic of China

      Xiaoyu Wang, Xueling Nie & Hongzhi Tang

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    1. Yuping Ma
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    2. Xiaoyu Wang
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    3. Xueling Nie
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    4. Zhan Zhang
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    7. Hongzhi Tang
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    Correspondence to Hongzhi Tang.

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    Yuping Ma and Xiaoyu Wang contributed equally to this work.

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    Ma, Y., Wang, X., Nie, X. et al. Microbial Degradation of Chlorogenic Acid by a Sphingomonas sp. Strain. Appl Biochem Biotechnol 179, 1381–1392 (2016). https://doi.org/10.1007/s12010-016-2071-2

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    • DOI: https://doi.org/10.1007/s12010-016-2071-2

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