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Cellulases: Classification, Methods of Determination and Industrial Applications

Applied Biochemistry and Biotechnology volume 179, pages 1346–1380 (2016)Cite this article

Abstract

Microbial cellulases have been receiving worldwide attention, as they have enormous potential to process the most abundant cellulosic biomass on this planet and transform it into sustainable biofuels and other value added products. The synergistic action of endoglucanases, exoglucanases, and β-glucosidases is required for the depolymerization of cellulose to fermentable sugars for transformation in to useful products using suitable microorganisms. The lack of a better understanding of the mechanisms of individual cellulases and their synergistic actions is the major hurdles yet to be overcome for large-scale commercial applications of cellulases. We have reviewed various microbial cellulases with a focus on their classification with mechanistic aspects of cellulase hydrolytic action, insights into novel approaches for determining cellulase activity, and potential industrial applications of cellulases.

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Affiliations

  1. Department of Microbial Biotechnology, Panjab University, Chandigarh, 160014, India

    Amita Sharma & Rupinder Tewari

  2. Department of Microbiology, Panjab University, Chandigarh, 160014, India

    Susheel Singh Rana & Sanjeev Kumar Soni

  3. Department of Biotechnology, D.A.V. College, Chandigarh, 160011, India

    Raman Soni

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  1. Amita Sharma
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  2. Rupinder Tewari
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  3. Susheel Singh Rana
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  4. Raman Soni
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  5. Sanjeev Kumar Soni
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Correspondence to Sanjeev Kumar Soni.

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Sharma, A., Tewari, R., Rana, S.S. et al. Cellulases: Classification, Methods of Determination and Industrial Applications. Appl Biochem Biotechnol 179, 1346–1380 (2016). https://doi.org/10.1007/s12010-016-2070-3

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  • DOI: https://doi.org/10.1007/s12010-016-2070-3

Keywords

  • Biofuel
  • Cellulose
  • Cellulases
  • Classification
  • Cellulase assays
  • Industrial applications