Applied Biochemistry and Biotechnology

, Volume 179, Issue 8, pp 1325–1335 | Cite as

Extraction of Oil from Flaxseed (Linum usitatissimum L.) Using Enzyme-Assisted Three-Phase Partitioning

  • Zhi-jian TanEmail author
  • Zi-zhen Yang
  • Yong-jian Yi
  • Hong-ying Wang
  • Wan-lai Zhou
  • Fen-fang Li
  • Chao-yun WangEmail author


In this study, enzyme-assisted three-phase partitioning (EATPP) was used to extract oil from flaxseed. The whole procedure is composed of two parts: the enzymolysis procedure in which the flaxseed was hydrolyzed using an enzyme solution (the influencing parameters such as the type and concentration of enzyme, temperature, and pH were optimized) and three-phase partitioning (TPP), which was conducted by adding salt and t-butanol to the crude flaxseed slurry, resulting in the extraction of flaxseed oil into alcohol-rich upper phase. The concentration of t-butanol, concentration of salt, and the temperature were optimized to maximize the extraction yield. Under optimized conditions of a 49.29 % t-butanol concentration, 30.43 % ammonium sulfate concentration, and 35 °C extraction temperature, a maximum extraction yield of 71.68 % was obtained. This simple and effective EATPP can be used to achieve high extraction yields and oil quality, and thus, it is potential for large-scale oil production.


Enzyme-assisted three-phase partitioning Flaxseed oil Enzyme kinetics GC–MS analysis 



This work was financially supported by the National Natural Science Foundation of China (21406262) and the Agricultural Science and Technology Innovation Program (ASTIP-IBFC07).


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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Zhi-jian Tan
    • 1
    Email author
  • Zi-zhen Yang
    • 2
  • Yong-jian Yi
    • 1
  • Hong-ying Wang
    • 1
  • Wan-lai Zhou
    • 1
  • Fen-fang Li
    • 2
  • Chao-yun Wang
    • 1
    Email author
  1. 1.Institute of Bast Fiber Crops, Chinese Academy of Agricultural SciencesChangshaChina
  2. 2.College of Chemistry and Chemical EngineeringCentral South UniversityChangshaChina

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