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Bovine Lactoferrampin, Human Lactoferricin, and Lactoferrin 1-11 Inhibit Nuclear Translocation of HIV Integrase

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Abstract

This study aimed to investigate fragments derived from human and bovine lactoferrins for ability to inhibit nuclear translocation of HIV-1 integrase. It was shown that human lactoferricin, human lactoferrin 1-11, and bovine lactoferrampin reduced nuclear distribution of HIV-1 integrase. Bovine lactoferrampin could inhibit both the activity and nuclear translocation of HIV-1 integrase. Human lactoferrampin, bovine lactoferricin, and bovine lactoferrin 1-11 had no effect on HIV-1 integrase nuclear translocation. Human lactoferrampin which inhibited the activity of integrase did not prevent its nuclear translocation. Human lactoferricin and lactoferrin 1-11 did not inhibit HIV-1 integrase nuclear translocation despite their ability to attenuate the enzyme activity. The discrepancy between the findings on reduction of HIV-1 activity and inhibition of nuclear translocation of HIV-1 integrase was due to the different mechanisms involved. A similar reasoning can also be applied to the different inhibitory potencies of the milk peptides on different HIV enzymes, i.e., nuclear translocation.

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Acknowledgments

We gratefully acknowledge the award of an HMRF research grant (reference no. 12110672) from Food and Health Bureau, Hong Kong, and a research grant (no. 81471927) from National Natural Science Foundation of China. We thank Professor S.A. Chow (School of Medicine, UCLA) for his gift of the recombinant HIV-1 integrase clone.

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Authors and Affiliations

  1. School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Hong Kong, China

    Winston Yan Wang, Jack Ho Wong, Denis Tsz Ming Ip, David Chi Cheong Wan, Randy Chifai Cheung & Tzi Bun Ng

  2. Shenzhen Research Institute, The Chinese University of Hong Kong, Hong Kong, China

    Jack Ho Wong & Tzi Bun Ng

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  1. Winston Yan Wang
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Correspondence to Jack Ho Wong or Tzi Bun Ng.

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Wang, W.Y., Wong, J.H., Ip, D.T.M. et al. Bovine Lactoferrampin, Human Lactoferricin, and Lactoferrin 1-11 Inhibit Nuclear Translocation of HIV Integrase. Appl Biochem Biotechnol 179, 1202–1212 (2016). https://doi.org/10.1007/s12010-016-2059-y

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