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Structural Analysis of the Lipopeptide Produced by the Bacillus subtilis Mutant R2-104 with Mutagenesis

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Abstract

The lipopeptide and its homologues are a kind of the five major biosurfactants with prominent interfacial and biological activities. A suite of mutagenesis method was adopted to expose a wild lipopeptide-producing strain Bacillus subtilis HSO121 to improve lipopeptide yield, and a stable mutant named R2-104 with a 2.0-fold production of lipopeptide was obtained. Compared to that of the wild strain HSO121, the lipopeptide produced by R2-104 showed a similar surface activity, but the course profiles of lipopeptide production during cultivation were different, with the peak yield of 500 mg at about 9 h by R2-104, and 400 mg at about 5 h by HSO121. The constituent abundance of the lipopeptide homologues produced by R2-104 was also different from that by HSO121. Combined methods of ESI-MS, GC-MS and MS-MS were applied for structural characterization of lipopeptide homologues, and it showed that the lipopeptides produced by R2-104 and HSO121 were attributed to a surfactin family with different constituents. The dominant constituent of the surfactin family produced by R2-104 was anteiso C15-surfactin with a relative content of 43.8 %, while the dominant one produced by HSO121was iso C14-surfactin with a relative content of 33.1 %.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (Grant No. 21203063 and 51574125) and the 863 Program (Grant No. 2013AA064403).

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Authors and Affiliations

  1. State Key Laboratory of Bioreactor Engineering and Institute of Applied Chemistry, East China University of Science and Technology, Shanghai, 200237, People’s Republic of China

    Yong Meng, Wei Zhao, Jia You, Hong-Ze Gang, Jin-Feng Liu, Shi-Zhong Yang, Ru-Qiang Ye & Bo-Zhong Mu

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  1. Yong Meng
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Correspondence to Bo-Zhong Mu.

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Meng, Y., Zhao, W., You, J. et al. Structural Analysis of the Lipopeptide Produced by the Bacillus subtilis Mutant R2-104 with Mutagenesis. Appl Biochem Biotechnol 179, 973–985 (2016). https://doi.org/10.1007/s12010-016-2044-5

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