Abstract
The use of residues from the industrial processing of palm oil as carbon source and inducer for microbial lipase production can be a way to add value to such residues and to contribute to reduced enzyme costs. The aim of this work was to investigate the feasibility of using palm oil industrial waste as feedstock for lipase production in different cultivation systems. Evaluation was made of lipase production by a selected strain of Aspergillus niger cultivated under solid-state (SSF) and submerged fermentation (SmF). Lipase activity levels up to 15.41 IU/mL were achieved under SSF. The effects of pH and temperature on the lipase activity of the SSF extract were evaluated using statistical design methodology, and maximum activities were obtained between pH 4.0 and 6.5 and at temperatures between 37 and 55 °C. This lipase presented good thermal stability up to 60 °C and higher specificity towards long carbon chain substrates. The results demonstrate the potential application of palm oil industrial residues for lipase production and contribute to the technological advances needed to develop processes for industrial enzymes production.
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Acknowledgments
The authors thank the Brazilian agencies FAPESP (project no. 2013/20826-0), CAPES, and CNPq for financial support, and the staff of EMBRAPA for technical assistance. We are grateful to F.M. da Cunha for internal review of this paper, and T.F.C. Salum and E.M. Penha for their fruitful collaboration in this research project. The research was also funded by EMBRAPA under grant no. 02.11.07.012.00.00.
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Silveira, E.A., Tardioli, P.W. & Farinas, C.S. Valorization of Palm Oil Industrial Waste as Feedstock for Lipase Production. Appl Biochem Biotechnol 179, 558–571 (2016). https://doi.org/10.1007/s12010-016-2013-z
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DOI: https://doi.org/10.1007/s12010-016-2013-z