Abstract
A laboratory-scale anoxic–oxic sequential reactor system was seeded with acclimatized mixed microbial consortium for the treatment of common effluent treatment plant (CETP) wastewater having 7000–7400 mg L−1 of COD and 3000–3400 mg L−1 of BOD. Initially, CETP wastewater was treated under anoxic reactor at 5000 mg L−1 of MLSS concentrations, 5.26 ± 0.27 kg COD m−3 day−1 of organic loading rate (OLR) and 36 h of hydraulic retention time (HRT). Further, the effluent of anoxic reactor was treated in oxic reactor with an OLR of 6.6 ± 0.31 kg COD m−3 day−1 and 18 h HRT. Maximum color and COD removal were found to be 72 and 85 % at total HRT of 2.25 days under anoxic–oxic sequential reactor at 37 °C and pH 7.0. The UV–VIS, FTIR, NMR and GCMS studies showed that majority of peaks observed in untreated wastewater were either shifted or disappeared after sequential treatment. Phytotoxicity study with the seeds of Vigna radiata and Triticum aestivum showed more sensitivity toward the CETP wastewater, while the products obtained after sequential treatment does not have any inhibitory effects. The results demonstrated that the anoxic–oxic reactor fed with bacterial consortium VN11 could bring about efficient bioremediation of industrial wastewaters.
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Acknowledgments
Authors gratefully acknowledge the Department of Biotechnology (DBT), New Delhi for the research grant (grant No. BT/PR-11724/BCE/08/719/2008) and the Government of India for the financial support during the study. The authors are thankful to Gujarat State Biotechnology Mission (GSBTM), Gandhinagar, Gujarat, India for providing facilities for DNA sequencing of pure cultures.
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ESM 1
Fig 1 Effect of (a) F/M ratio on sludge volume index (SVI) (b) pH of the substrate entering to the oxic reactor and (c) wastewater DO level for the treatment of wastewater by consortium VN11. (DOC 104 kb)
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Table 1 Evaluation of degradation at sequential anoxic-oxic bioreactor. (DOC 27 kb)
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Fig. 2 UV-VIS spectrum of wastewater before and after treatment by sequential anoxic-oxic bioreactor with the help of consortium VN11. (DOC 43 kb)
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Fig. 3 FTIR spectrum of wastewater (a) before and (b) after treatment by sequential anoxic-oxic bioreactor with the help of consortium VN11. (DOC 284 kb)
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Fig. 4 NMR spectrum of wastewater (a) before and (b) after treatment by sequential anoxic-oxic bioreactor with the help of consortium VN11. (DOC 117 kb)
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Table 2 List of intermediates identified on the basis of GC-MS (DOC 287 kb)
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Fig. 5 Scanning Electron Microscopic analysis of sludge of wastewater after anoxic stage treatment (a) 3500× magnification (b) 10000× magnification. (DOC 1558 kb)
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Fig. 6 Scanning Electron Microscopic analysis of sludge from oxic reactor during wastewater treatment with the sequential anoxic-oxic bioreactor by consortium VN11 at different magnification (a) 10000× magnification and (b) 15000× magnification. (DOC 1841 kb)
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Chattaraj, S., Purohit, H.J., Sharma, A. et al. Treatment of Common Effluent Treatment Plant Wastewater in a Sequential Anoxic–Oxic Batch Reactor by Developed Bacterial Consortium VN11. Appl Biochem Biotechnol 179, 514–529 (2016). https://doi.org/10.1007/s12010-016-2010-2
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DOI: https://doi.org/10.1007/s12010-016-2010-2