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The Bioconversion of Pretreated Cashew Apple Bagasse into Ethanol by SHF and SSF Processes

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Abstract

Ethanol production from acidic-alkaline pretreated cashew apple bagasse (CAB-OH) was investigated using separated hydrolysis and fermentation (SHF) and simultaneous saccharification and fermentation (SSF) processes. First, a screening of Kluyveromyces strains was conducted by SHF and a maximum ethanol concentration of 24.1 g L−1 was obtained using Kluyveromyces marxianus ATCC36907, which presented similar profiles when compared to results obtained by a Saccharomyces strain. The effect of temperature on ethanol production conducted by SHF using K. marxianus ATCC36907 was investigated, and the maximum ethanol yield (YE/G) was obtained at 40 °C (0.46 g g−1) using a synthetic medium. In the SHF using CAB-OH hydrolysate, the maximum ethanol concentration obtained was 24.9 g L−1, 5.92 g L−1 h−1 of productivity, and ethanol yield of 0.43 g g−1 at 40 °C. Afterwards, K. marxianus ATCC36907 was used in the bioconversion of CAB-OH by SSF, and an ethanol concentration of 41.41 ± 0.2 g L−1 was obtained using 10 % CAB-OH at 40 °C, 150 rpm and 24 h, resulting in a YʹE/G of 0.50 gE gG −1 and an efficiency of 98.4 %, in the process conducted with cellobiase supplementation. SHF and SSF processes using CAB-OH and K. marxianus ATCC36907 can be used to ethanol production, but the SSF process required only one step to achieve the same production.

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Acknowledgments

The authors would like to thank the Brazilian research funding agencies ANP, CAPES, and CNPq (Federal). Saccharomyces sp. 1238 was kindly donated by the Laboratory of Biotechnology Process, located at the Federal University of Pernambuco, Recife, Brazil. We also thank Dr. Sandra Ceccato-Antonini from the Laboratory of Agricultural Microbiology, UFSCar – São Paulo – Brazil, for the Kluyveromyces marxianus CCA510 strain. In addition, the authors gratefully acknowledge Novozymes for donating the enzyme complex used in this study.

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  1. Departamento de Engenharia Química, Universidade Federal do Ceará, Campus do Pici, Bloco 709, 60455-760, Fortaleza, CE, Brazil

    Tigressa Helena S. Rodrigues, Emanuel Meneses de Barros, Jeferson de Sá Brígido, Winne M. da Silva, Maria Valderez P. Rocha & Luciana Rocha B. Gonçalves

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Rodrigues, T.H.S., de Barros, E.M., de Sá Brígido, J. et al. The Bioconversion of Pretreated Cashew Apple Bagasse into Ethanol by SHF and SSF Processes. Appl Biochem Biotechnol 178, 1167–1183 (2016). https://doi.org/10.1007/s12010-015-1936-0

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