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A Novel Optical Biosensing System Using Mach–Zehnder-Type Optical Waveguide for Influenza Virus Detection

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Abstract

In order to minimize the damage from viral epidemics, early detection of the causative agent of a viral epidemic and prevention of its immediate spread are urgent social demands. Therefore, in this study, we evaluated the utility of a Mach–Zehnder-type optical waveguide as a sensing device for influenza virus detection. However, it is impossible to detect a 100-nm-size virus using a sol-gel optical biosensor because sol-gel glass has a pore size of only a few nanometers, which makes it impossible for the virus to diffuse into the silica thin film. In order to construct the influenza-specific Mach–Zehnder optical biosensor for influenza detection, a stable antibody immobilization method with resulting high density on the sol-gel surface is strongly required. In this study, the sol-gel glass surface was modified with amino and carboxyl groups, and an anti-H1N1/HA1 antibody was covalently immobilized using a cross-linking agent. We successfully prepared a carboxyl-modified sol-gel surface, using NHS/EDC as the cross-linker, for antibody immobilization, and confirmed the detection of influenza virus using the antibody-immobilized sol-gel glass. After treatment with a 100 μg/mL influenza virus solution for 15 min, a peak wavelength shift (~24 nm) was observed in the output light spectrum.

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Authors and Affiliations

  1. Tenure-Track Program for Innovation Research, University of Fukui, Fukui, Japan

    Hiroaki Sakamoto

  2. Department of Applied Chemistry and Biotechnology, Graduate School of Engineering, University of Fukui, Fukui, Japan

    Yuma Minpou

  3. Department of Frontier Fiber Technology and Science, Graduate School of Engineering, University of Fukui, Fukui, Japan

    Takayuki Sawai & Shin-ichiro Suye

  4. Optoelectonic Engineering, School of Systems Engineering, Kochi University of Technology, Kochi, Japan

    Yasufumi Enami

  5. Research and Education Program for Life Science, University of Fukui, Bunkyo 3-9-1, Fukui, 910-8507, Japan

    Hiroaki Sakamoto, Takayuki Sawai & Shin-ichiro Suye

Authors
  1. Hiroaki Sakamoto
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  2. Yuma Minpou
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  3. Takayuki Sawai
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  4. Yasufumi Enami
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  5. Shin-ichiro Suye
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Correspondence to Shin-ichiro Suye.

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Sakamoto, H., Minpou, Y., Sawai, T. et al. A Novel Optical Biosensing System Using Mach–Zehnder-Type Optical Waveguide for Influenza Virus Detection. Appl Biochem Biotechnol 178, 687–694 (2016). https://doi.org/10.1007/s12010-015-1902-x

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  • DOI: https://doi.org/10.1007/s12010-015-1902-x

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