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Microbial Selenium Nanoparticles (SeNPs) and Their Application as a Sensitive Hydrogen Peroxide Biosensor

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Abstract

The cell-free extract, a crude enzyme (cytosolic and membrane fraction) obtained from an environmental isolate, Bacillus pumilus sp. BAB-3706 worked as excellent in reducing as well as stabilizing agent and facilitated the formation of stable colloidal selenium nanoparticles (SeNPs). Resulting nanoparticles were characterized using UV-vis spectrophotometer, TEM, EDAX, FT-IR and XRD, respectively. A working electrode was modified by coating the surface of indium tin oxide (ITO) with colloidal SeNPs. Successive additions of H2O2 (100 to 600 μM) in conventional three electrodes system, cyclic voltammeter with potential scan rate 25.0 mV/s, in 0.1 M phosphate buffer solution (PBS) yielded increase in current. A perpetual amperometric response at fixed potential (−1.0 V) and at selected time interval of 100 s showed different magnitude of current at every addition of H2O2. The linear range of detection of H2O2 was from 5 to 600 mM (R 2 = 0.9965), while the calculated limit of detection was found to be 3.00 μM. The current study suggested that microbial SeNPs can be used for fabrication of low cost, sensitive H2O2 biosensor.

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Acknowledgments

KSP is grateful to Mr. Vipul J. Patel, senior scientific officer at DST (Department Science and Technology) sponsored SICART (Sophisticated Instrumentation Center for Applied Research and Testing) Anand, Gujarat, India, for his help in the analysis of samples.

Conflict of Interest

The authors declare that they have no conflict of interests.

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Authors and Affiliations

  1. Department of Environmental Studies, Faculty of Science, The M. S. University of Baroda, Vadodara, 390002, Gujarat, India

    Kumar Suranjit Prasad

  2. Department of Chemistry, Sardar Patel University, Anand, 388120, Gujarat, India

    Jayraj V. Vaghasiya & Saurabh S. Soni

  3. ARIBAS, New Vallabh Vidyanagar, Anand, 388120, Gujarat, India

    Jitesh Patel & Rinkesh Patel

  4. Department of Botany, B. R. A. Bihar University, Muzaffarpur, 842002, Bihar, India

    Madhu Kumari

  5. Gujarat Biodiversity Gene Bank, Udyogbhavan, Gandhinagar, Gujarat, 382010, India

    Falguni Jasmani

  6. National Chemical Laboratory, Pune, 411008, India

    Kaliaperumal Selvaraj

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  1. Kumar Suranjit Prasad
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Correspondence to Kumar Suranjit Prasad.

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Prasad, K.S., Vaghasiya, J.V., Soni, S.S. et al. Microbial Selenium Nanoparticles (SeNPs) and Their Application as a Sensitive Hydrogen Peroxide Biosensor. Appl Biochem Biotechnol 177, 1386–1393 (2015). https://doi.org/10.1007/s12010-015-1814-9

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  • DOI: https://doi.org/10.1007/s12010-015-1814-9

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