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Assessment of a Novel Algal Strain Chlamydomonas debaryana NIREMACC03 for Mass Cultivation, Biofuels Production and Kinetic Studies

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Abstract

A novel microalgae strain Chlamydomonas debaryana (KJ210856) was isolated from a freshwater lake of Punjab, India, and cultivated considering climatic sustainability and inherent adaptability concern. C. debaryana was grown in a 30-L indoor photobioreactor to study the mass cultivation prospect and biofuel potential. Physicochemical characterization of biomass and the lipid was performed with effect to nitrogen stress. It showed a higher biomass yield (1.58 ± 0.02 g L−1, dry weight) and twofold increase in lipid yield (552.78 ± 9 mg L−1) with 34.2 ± 0.19 % lipid content under nitrogen deficient condition. Strikingly, increase in triglycerides achieved with nitrogen depletion containing over 96 % of total fatty acids (C 14, C 16, and C 18). Proximate and ultimate analysis suggested the presence of relatively higher volatile matter and carbon-hydrogen ratio. Furthermore, lower moisture and ash content signified C. debaryana biomass has promising features towards biofuel applications. The pyrolytic behavior of the whole biomass was also studied using thermogravimetric analyzer (TGA) and kinetic parameters were estimated using different methods. Promising growth rate and lipid yield leading to feasible biofuel feed stock production in indoor photobioreactor along with autosediment potential of cells validates C. debaryana NIREMACC03, a potential strain for mass cultivation.

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Acknowledgments

The first author gratefully acknowledges the Bioenergy Promotion Fellowship awarded by SSS-NIRE for conducting this research.

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Authors and Affiliations

  1. Sardar Swaran Singh National Institute of Renewable Energy, 12KM Stone, Jalandhar Kapurthala Road, Kapurthala, Punjab, 144 601, India

    Sanjeev Mishra, Neetu Singh & Anil Kumar Sarma

  2. Dr B R Ambedkar National Institute of Technology, Jalandhar, Punjab, 144011, India

    Neetu Singh

Authors
  1. Sanjeev Mishra
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  2. Neetu Singh
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  3. Anil Kumar Sarma
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Corresponding author

Correspondence to Sanjeev Mishra.

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Highlights

• C. debaryana NIREMACC03 isolated and identified through 18S rRNA gene sequencing.

• Uptake of twofold increase in lipid productivity during mass cultivation.

• Complete physicochemical characterization of C. debaryana and fatty acid profile.

• Induction of saturated fatty acids achieved with effect to nitrogen stress.

• Activation energy and kinetic parameters of the biomass evaluated using TGA.

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Mishra, S., Singh, N. & Sarma, A.K. Assessment of a Novel Algal Strain Chlamydomonas debaryana NIREMACC03 for Mass Cultivation, Biofuels Production and Kinetic Studies. Appl Biochem Biotechnol 176, 2253–2266 (2015). https://doi.org/10.1007/s12010-015-1714-z

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  • DOI: https://doi.org/10.1007/s12010-015-1714-z

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