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IL-17A Autoantibody Induced by Recombinant Mycobacterium smegmatis Expressing Ag85A-IL-17A Fusion Protein

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Abstract

Interleukin-17A is a newly described proinflammatory cytokine, which plays important roles in autoimmune diseases as well as asthma. In current work, we constructed a recombinant plasmid pMFA42S-Ag85a-IL-17a by inserting fusion gene Ag85a-IL-17a into shuttle vector pMFA42S, which was transformed to Mycobacterium smegmatis by electroporation to obtain recombinant M. smegmatis named rMS-Ag85a-IL-17a. The comparison of growth pattern between M. smegmatis and rMS-Ag85a-IL-17a suggested fusion gene had no significant influence on the growth of strains, and rMS-Ag85a-IL-17a expressed fusion protein Ag85A-IL-17A which had good immunogenicity revealed by Western blot. M. smegmatis and rMS-Ag85a-IL-17a were performed to intranasally immunize mice; then, antibody response in sera was detected by enzyme-linked immunosorbent assay. Our findings demonstrated that rMS-Ag85a-IL-17a could induce specific IL-17A autoantibody in mice, which laid the foundation for further study.

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Acknowledgments

This work was supported by a grant (No. 81172887) from the National Natural Science Foundation of China.

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Authors and Affiliations

  1. Department of Pediatrics, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital, Shanghai, China

    Ling Chen, Sheng Guo, Liangxia Wu & Jianhua Zhang

  2. Scientific Institute, Shanghai Public Health Clinical Center Affiliated to Fudan University, Shanghai, China

    Xiaoyong Fan, Hui Ma, Kang Wu & Juan Wu

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  1. Ling Chen
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  2. Sheng Guo
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  3. Liangxia Wu
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  4. Xiaoyong Fan
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  6. Kang Wu
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  7. Juan Wu
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  8. Jianhua Zhang
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Correspondence to Jianhua Zhang.

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Chen, L., Guo, S., Wu, L. et al. IL-17A Autoantibody Induced by Recombinant Mycobacterium smegmatis Expressing Ag85A-IL-17A Fusion Protein. Appl Biochem Biotechnol 176, 2018–2026 (2015). https://doi.org/10.1007/s12010-015-1697-9

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  • DOI: https://doi.org/10.1007/s12010-015-1697-9

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