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Biodegradation of Benzene, Toluene, Ethylbenzene, and o-, m-, and p-Xylenes by the Newly Isolated Bacterium Comamonas sp. JB

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Abstract

A bacterium designated strain JB, able to degrade six benzene, toluene, ethylbenzene, and o-, m-, and p-xylene (BTEX) compounds, was isolated from petroleum-contaminated soil. Taxonomic analyses showed that the isolate belonged to Comamonas, and until now, the genus Comamonas has not included any known BTEX degraders. The BTEX biodegradation rate was slightly low on the mineral salt medium (MSM), but adding a small amount of yeast extract greatly enhanced the biodegradation. The relationship between specific degradation rate and individual BTEX was described well by Michaelis-Menten kinetics. The treatment of petrochemical wastewater containing BTEX mixture and phenol was shown to be highly efficient by BTEX-grown JB. In addition, toxicity assessment indicated the treatment of the petrochemical wastewater by BTEX-grown JB led to less toxicity than untreated wastewater.

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Acknowledgments

This work was supported by grants from the Science and Technology Project of Liaoning Province (2014203006), Ocean & Fisheries Project of Liaoning Province (201301), Public Science and Technology Research Funds Projects of Ocean (201205012–7), and Science and Technology Project of Dalian City (2012J21DW029).

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Correspondence to Bei Jiang.

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Jiang, B., Zhou, Z., Dong, Y. et al. Biodegradation of Benzene, Toluene, Ethylbenzene, and o-, m-, and p-Xylenes by the Newly Isolated Bacterium Comamonas sp. JB. Appl Biochem Biotechnol 176, 1700–1708 (2015). https://doi.org/10.1007/s12010-015-1671-6

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  • DOI: https://doi.org/10.1007/s12010-015-1671-6

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