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Biodegradation of Benzene, Toluene, Ethylbenzene, and o-, m-, and p-Xylenes by the Newly Isolated Bacterium Comamonas sp. JB

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A bacterium designated strain JB, able to degrade six benzene, toluene, ethylbenzene, and o-, m-, and p-xylene (BTEX) compounds, was isolated from petroleum-contaminated soil. Taxonomic analyses showed that the isolate belonged to Comamonas, and until now, the genus Comamonas has not included any known BTEX degraders. The BTEX biodegradation rate was slightly low on the mineral salt medium (MSM), but adding a small amount of yeast extract greatly enhanced the biodegradation. The relationship between specific degradation rate and individual BTEX was described well by Michaelis-Menten kinetics. The treatment of petrochemical wastewater containing BTEX mixture and phenol was shown to be highly efficient by BTEX-grown JB. In addition, toxicity assessment indicated the treatment of the petrochemical wastewater by BTEX-grown JB led to less toxicity than untreated wastewater.

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  1. Assinder, S. J., & Williams, P. A. (1990). The TOL plasmids: determinants of the catabolism of toluene and the xylenes. Advances in Microbial Physiology, 31, 1–69.

    Article  CAS  Google Scholar 

  2. Chang, J. H., Chang, Y. K., Ryu, H. W., & Chang, H. N. (2000). Desulfurization of light gas oil in immobilized-cell systems of Gordona sp. CYKS1 and Nocardia sp. CYKS2. FEMS Microbiology Letters, 82, 309–312.

    Article  Google Scholar 

  3. Rahul, Mathur, A. K., & Balomajumder, C. (2013). Biological treatment and modeling aspect of BTEX abatement process in a biofilter. Bioresource Technology, 142, 9–17.

    Article  CAS  Google Scholar 

  4. Jennifer, V. L., & Andrew, J. D. (2008). Kinetics and interactions of BTEX compounds during degradation by a bacterial consortium. Process Biochemistry, 43, 1068–1076.

    Article  Google Scholar 

  5. Costa, A. S., Romão, L. P., Araújo, B. R., Lucas, S. C., Maciel, S. T., Wisniewski, A., Jr., & Alexandre, M. R. (2012). Environmental strategies to remove volatile aromatic fractions (BTEX) from petroleum industry wastewater using biomass. Bioresource Technology, 105, 31–39.

    Article  CAS  Google Scholar 

  6. Dean, B. J. (1985). Recent findings on the genetic toxicology of benzene, toluene, xylenes and phenols. Mutation Research, 154, 153–181.

    Article  CAS  Google Scholar 

  7. Kim, J. M., Le, N. T., Chung, B. S., Park, J. H., Bae, J. W., Madsen, E. L., & Jeon, C. O. (2008). Influence of soil components on the biodegradation of benzene, toluene, ethylbenzene, and o-, m-, and p-xylenes by the newly isolated bacterium Pseudoxanthomonas spadix BD-a59. Applied and Environmental Microbiology, 74, 7313–7320.

    Article  CAS  Google Scholar 

  8. Yadav, J. S., & Reddy, C. A. (1993). Applied and Environmental Microbiology, 59, 756–762.

    CAS  Google Scholar 

  9. Chakraborty, R., O’Connor, S. M., Chan, E., & Coates, J. D. (2005). Anaerobic degradation of benzene, toluene, ethylbenzene, and xylene compounds by Dechloromonas strain RCB. Applied and Environmental Microbiology, 71, 8649–8655.

    Article  CAS  Google Scholar 

  10. Kim, D., Kim, Y. S., Kim, S. K., Kim, S. W., Zylstra, G. J., Kim, Y. M., & Kim, E. (2002). Monocyclic aromatic hydrocarbon degradation by Rhodococcus sp. strain DK17. Applied and Environmental Microbiology, 68, 3270–3278.

    Article  CAS  Google Scholar 

  11. Shinoda, Y., Sakai, Y., Uenishi, H., Uchihashi, Y., Hiraishi, A., Yukawa, H., Yurimoto, H., & Kato, N. (2004). Aerobic and anaerobic toluene degradation by a newly isolated denitrifying bacterium, Thauera sp. strain DNT-1. Applied and Environmental Microbiology, 70, 1385–1392.

    Article  CAS  Google Scholar 

  12. Zylstra, G. J., & Kim, E. (1997). Aromatic hydrocarbon degradation by Sphingomonas yanoikuyae B1. Journal of Industrial Microbiology and Biotechnology, 19, 408–414.

    Article  CAS  Google Scholar 

  13. Jin, H. M., Choi, E. J., & Jeon, C. O. (2013). Isolation of a BTEX-degrading bacterium, Janibacter sp. SB2, from a sea-tidal flat and optimization of biodegradation conditions. Bioresource Technology, 145, 57–64.

    Article  CAS  Google Scholar 

  14. Kim, J. M., & Jeon, C. O. (2009). Isolation and characterization of a new benzene, toluene, and ethylbenzene degrading bacterium, Acinetobacter sp. B113. Current Microbiology, 58, 70–75.

    Article  CAS  Google Scholar 

  15. Shi, S. N., Qu, Y. Y., Ma, F., & Zhou, J. T. (2014). Bioremediation of coking wastewater containing carbazole, dibenzofuran, dibenzothiphene and naphthalene by a naphthalene-cultivated Arthrobacter sp. W1. Bioresource Technology, 164, 28–33.

    Article  CAS  Google Scholar 

  16. Prenafeta-Boldú, F. X., Vervoort, J., Grotenhuis, J. T., & Van Groenestijn, J. W. (2002). Substrate interactions during the biodegradation of benzene, toluene, ethylbenzene, and xylene (BTEX) hydrocarbons by the fungus Cladophialophora sp. strain T1. Applied and Environmental Microbiology, 8, 2660–2665.

    Article  Google Scholar 

  17. Hatzinger, P. B., McCalay, K., Vainberg, S., Tugusheva, M., Condee, C. W., & Steffan, R. J. (2001). Biodegradation of methyl tert-butyl ether by a pure bacterial culture. Applied and Environmental Microbiology, 67, 5601–5607.

    Article  CAS  Google Scholar 

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This work was supported by grants from the Science and Technology Project of Liaoning Province (2014203006), Ocean & Fisheries Project of Liaoning Province (201301), Public Science and Technology Research Funds Projects of Ocean (201205012–7), and Science and Technology Project of Dalian City (2012J21DW029).

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Correspondence to Bei Jiang.

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Jiang, B., Zhou, Z., Dong, Y. et al. Biodegradation of Benzene, Toluene, Ethylbenzene, and o-, m-, and p-Xylenes by the Newly Isolated Bacterium Comamonas sp. JB. Appl Biochem Biotechnol 176, 1700–1708 (2015).

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